Implantable aptamer-field-effect transistor neuroprobes for in vivo neurotransmitter monitoring.
Implantable aptamer-field-effect transistor neuroprobes for in vivo neurotransmitter monitoring.
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DOI：
10.1126/sciadv.abj7422
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发表时间：
2021-11-26
期刊：
影响因子：
13.6
通讯作者：
Andrews AM
中科院分区：
文献类型：
--
作者：
Zhao C;Cheung KM;Huang IW;Yang H;Nakatsuka N;Liu W;Cao Y;Man T;Weiss PS;Monbouquette HG;Andrews AM
用于体内神经递质监测的可植入适体晶体管探针促进了大脑活动记录。虽然用于监测体内电生理学的工具已经被广泛开发，但神经化学记录技术仍然有限。然而，通过神经递质的化学通讯在大脑信息处理中起着核心作用。我们开发了用于监测神经递质的可植入适体场效应晶体管（FET）神经探针。使用高通量微机电系统（MEMS）技术制造神经探针，其中在4英寸Si晶片上制造150个具有150或50 μm宽度和厚度的探针。采用溶胶-凝胶法制备了In_2O_3半导体纳米薄膜场效应晶体管。In 2 O3表面与合成的寡核苷酸受体（适体）结合，以识别和检测神经递质5-羟色胺。适体-FET神经探针使脑组织中的飞摩尔5-羟色胺检测限具有最小的生物污染。在体内检测刺激的5-羟色胺释放。这项研究通过将这些适体-FET传感器与其他类型的基于Si的植入式探针相结合，以高时空分辨率进行综合神经活动记录，从而促进我们对大脑功能的理解。
Implantable aptamer transistor probes for in vivo neurotransmitter monitoring advance brain activity recording. While tools for monitoring in vivo electrophysiology have been extensively developed, neurochemical recording technologies remain limited. Nevertheless, chemical communication via neurotransmitters plays central roles in brain information processing. We developed implantable aptamer–field-effect transistor (FET) neuroprobes for monitoring neurotransmitters. Neuroprobes were fabricated using high-throughput microelectromechanical system (MEMS) technologies, where 150 probes with shanks of either 150- or 50-μm widths and thicknesses were fabricated on 4-inch Si wafers. Nanoscale FETs with ultrathin (~3 to 4 nm) In2O3 semiconductor films were prepared using sol-gel processing. The In2O3 surfaces were coupled with synthetic oligonucleotide receptors (aptamers) to recognize and to detect the neurotransmitter serotonin. Aptamer-FET neuroprobes enabled femtomolar serotonin detection limits in brain tissue with minimal biofouling. Stimulated serotonin release was detected in vivo. This study opens opportunities for integrated neural activity recordings at high spatiotemporal resolution by combining these aptamer-FET sensors with other types of Si-based implantable probes to advance our understanding of brain function.
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影响因子：
4
作者：
Kim, B. J.;Kuo, J. T. W.;Meng, E.
通讯作者：
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影响因子：
64.8
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影响因子：
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影响因子：
28.1
作者：
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Tian, Bozhi