Integrated microfluidic systems with sample preparation and nucleic acid amplification.
Integrated microfluidic systems with sample preparation and nucleic acid amplification.
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DOI：
10.1039/c9lc00389d
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发表时间：
2019-09-07
期刊：
影响因子：
6.1
通讯作者：
Mu Y
中科院分区：
文献类型：
--
作者：
Yin J;Suo Y;Zou Z;Sun J;Zhang S;Wang B;Xu Y;Darland D;Zhao JX;Mu Y
快速、高效和准确的核酸分子检测对于疾病和病原体的筛查非常重要，但仍然是护理点（POC）治疗的限制因素。微流体系统的特征在于快速、集成、小型化的特征，其为核酸分子的定性和定量检测提供了有效的平台。核酸检测过程主要包括样品制备和目标分子扩增。鉴于迄今为止理论研究和技术创新的进步，与微流体系统集成的核酸提取和扩增得到了迅速发展。这篇综述的主要目标是概述当前用于微流体系统中核酸检测的方法。第二个目标是确定新的方法，这将有助于塑造核酸检测和微流体交叉领域的未来趋势，特别是在增加疾病和病原体检测以改善诊断和治疗方面。
Rapid, efficient and accurate nucleic acid molecule detection is important in the screening of diseases and pathogens, yet remains a limiting factor at point of care (POC) treatment. Microfluidic systems are characterized by fast, integrated, miniaturized features which provide an effective platform for qualitative and quantitative detection of nucleic acid molecules. The nucleic acid detection process mainly includes sample preparation and target molecule amplification. Given the advancements in theoretical research and technological innovations to date, nucleic acid extraction and amplification integrated with microfluidic systems has advanced rapidly. The primary goal of this review is to outline current approaches used for nucleic acid detection in the context of microfluidic systems. The secondary goal is to identify new approaches that will help shape future trends at the intersection of nucleic acid detection and microfluidics, particularly with regard to increasing disease and pathogen detection for improved diagnosis and treatment.
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