Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.
Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.
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DOI：
10.3390/mi8090266
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发表时间：
2017-08-30
期刊：
影响因子：
3.4
通讯作者：
Hamid NHB
中科院分区：
文献类型：
--
作者：
Basha IHK;Ho ETW;Yousuff CM;Hamid NHB
高度敏感和特异的病原诊断对于正确及时治疗人类传染病，特别是强毒株至关重要。即时病原体诊断对于管理疾病爆发以及日常医疗保健环境可以提供巨大帮助。使用分子方法可以高度特异性地识别传染性病原体。近年来大量的微流体创新使得开发便携式、稳健、准确和灵敏的基因组诊断设备以在护理点部署变得越来越可行。然而，提高处理时间、多重检测、灵敏度和检测限、特异性以及在资源有限的环境中易于部署是持续存在的挑战。这篇综述概述了微流控基因组诊断和设备的最新技术，重点是将它们集成到芯片实验室中，这将导致高灵敏度和特异性的多重护理点设备的开发。
Highly sensitive and specific pathogen diagnosis is essential for correct and timely treatment of infectious diseases, especially virulent strains, in people. Point-of-care pathogen diagnosis can be a tremendous help in managing disease outbreaks as well as in routine healthcare settings. Infectious pathogens can be identified with high specificity using molecular methods. A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point of care. However, improving processing time, multiplexed detection, sensitivity and limit of detection, specificity, and ease of deployment in resource-limited settings are ongoing challenges. This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care devices of high sensitivity and specificity.
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