MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast.
MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast.
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MOR 1是微管相关蛋白MAP 215家族的一员，在植物细胞中建立间期皮层微管阵列是必需的。在这里，我们表明，MOR 1结合微管在体内，定位于两个皮层微管和重叠的微管在成膜体的地区。我们证明了MOR 1在胞质分裂中的重要作用，通过与MOR 1的胞质分裂缺陷gem 1 -1突变的遗传互补。gem 1 -1和一个T-DNA插入等位基因gem 1 -2的表型分析证实MOR 1/GEM 1对胞质分裂的规则模式是必不可少的。两种突变gem 1 -1和gem 1 -2都导致MOR 1/GEM 1的截短。此外，在两个突变体中不存在的C-末端结构域在体外结合微管。这些数据表明MOR 1/GEM 1在细胞动力学成膜体中起着重要作用。
MOR1 is a member of the MAP215 family of microtubule-associated proteins and is required to establish interphase cortical microtubule arrays in plant cells. Here we show that MOR1 binds microtubules in vivo, localising to both cortical microtubules and to areas of overlapping microtubules in the phragmoplast. We demonstrate an essential role for MOR1 in cytokinesis through genetic complementation of the cytokinesis defective gem1-1 mutation with MOR1. Phenotypic analysis of gem1-1 and a T-DNA insertion allele gem1-2 confirm that MOR1/GEM1 is essential for regular patterns of cytokinesis. Both mutations, gem1-1 and gem1-2, cause the truncation of MOR1/GEM1. Moreover, the C-terminal domain absent in both mutants binds microtubules in vitro. These data demonstrate that MOR1/GEM1 plays an essential role in the cytokinetic phragmoplast.
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影响因子：
4.9
作者：
Igarashi, H;Orii, H;Sonobe, S
通讯作者：
Sonobe, S
影响因子：
7.4
作者：
Park, SK;Twell, D
通讯作者：
Twell, D
影响因子：
7.8
作者：
VASQUEZ, RJ;GARD, DL;CASSIMERIS, L
通讯作者：
CASSIMERIS, L
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1083/jcb.139.5.1271
发表时间：
1997-12-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Wang PJ;Huffaker TC
通讯作者：
Huffaker TC