Fluorescent imaging of single nanoparticles and viruses on a smart phone.
Fluorescent imaging of single nanoparticles and viruses on a smart phone.
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DOI：
10.1021/nn4037706
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发表时间：
2013-10-22
期刊：
影响因子：
17.1
通讯作者：
Ozcan, Aydogan
中科院分区：
文献类型：
--
作者：
Wei, Qingshan;Qi, Hangfei;Luo, Wei;Tseng, Derek;Ki, So Jung;Wan, Zhe;Gorocs, Zoltan;Bentolila, Laurent A.;Wu, Ting-Ting;Sun, Ren;Ozcan, Aydogan
纳米尺度物体的光学成像，无论是基于散射还是基于荧光，都是一项具有挑战性的任务，因为在亚波长尺寸下检测信噪比和对比度降低。在这里，我们报告了一种安装在智能手机上的野外便携式荧光显微镜平台，该平台使用轻巧紧凑的光学机械附件连接到手机现有的相机模块，用于对单个纳米颗粒和病毒进行成像。这种手持式荧光成像装置利用(i)一个紧凑的450 nm激光二极管，在样品平面上产生倾斜激发，入射角为~75°，（ii）一个长通薄膜干涉滤光片，以抑制散射激发光，（iii）一个外部透镜，产生2倍的光学放大倍率，（iv）一个用于焦点调整的平移台。我们通过检测分离的100 nm荧光颗粒以及荧光标记的单个人巨细胞病毒，测试了该智能手机支持的显微镜平台的成像性能。每个检测到的纳米物体在手机平台上的大小是通过相同样品的扫描电子显微镜图像进行验证的。这种附着在手机上的野外便携式荧光显微镜仅重约186克，可用于对包括各种细菌和病毒在内的亚波长物体进行特定和敏感成像，因此可以为在野外环境中实践纳米技术以及在偏远和资源有限的环境中进行病毒载量测量和其他生物医学测试提供宝贵的平台。
Optical imaging of nanoscale objects, whether it is based on scattering or fluorescence, is a challenging task due to reduced detection signal-to-noise ratio and contrast at subwavelength dimensions. Here, we report a field-portable fluorescence microscopy platform installed on a smart phone for imaging of individual nanoparticles as well as viruses using a lightweight and compact opto-mechanical attachment to the existing camera module of the cell phone. This hand-held fluorescent imaging device utilizes (i) a compact 450 nm laser diode that creates oblique excitation on the sample plane with an incidence angle of ~75°, (ii) a long-pass thin-film interference filter to reject the scattered excitation light, (iii) an external lens creating 2× optical magnification, and (iv) a translation stage for focus adjustment. We tested the imaging performance of this smart-phone-enabled microscopy platform by detecting isolated 100 nm fluorescent particles as well as individual human cytomegaloviruses that are fluorescently labeled. The size of each detected nano-object on the cell phone platform was validated using scanning electron microscopy images of the same samples. This field-portable fluorescence microscopy attachment to the cell phone, weighing only ~186 g, could be used for specific and sensitive imaging of subwavelength objects including various bacteria and viruses and, therefore, could provide a valuable platform for the practice of nanotechnology in field settings and for conducting viral load measurements and other biomedical tests even in remote and resource-limited environments.
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影响因子：
3.7
作者：
Haspot F;Lavault A;Sinzger C;Laib Sampaio K;Stierhof YD;Pilet P;Bressolette-Bodin C;Halary F
通讯作者：
Halary F
影响因子：
3.7
作者：
Smith ZJ;Chu K;Espenson AR;Rahimzadeh M;Gryshuk A;Molinaro M;Dwyre DM;Lane S;Matthews D;Wachsmann-Hogiu S
通讯作者：
Wachsmann-Hogiu S
影响因子：
6.1
作者：
Gallegos, Dustin;Long, Kenneth D.;Cunningham, Brian T.
通讯作者：
Cunningham, Brian T.
DOI：
10.1073/pnas.0611503104
发表时间：
2007-02-20
影响因子：
11.1
作者：
Mukhopadhyay, Samrat;Krishnan, Rajaraman;Deniz, Ashok A.
通讯作者：
Deniz, Ashok A.
影响因子：
3.7
作者：
Granot Y;Ivorra A;Rubinsky B
通讯作者：
Rubinsky B