De Novo Transcriptome Assembly and Characterization of the Synthesis Genes of Bioactive Constituents in Abelmoschus esculentus (L.) Moench.
De Novo Transcriptome Assembly and Characterization of the Synthesis Genes of Bioactive Constituents in Abelmoschus esculentus (L.) Moench.
复制标题
黄秋葵生物活性成分合成基因的从头转录组组装和表征
作者：
Zhang C;Dong W;Gen W;Xu B;Shen C;Yu C
黄秋葵（秋葵或女士的手指）是一种具有很高营养价值的蔬菜，以及具有一定的药用价值。它被广泛用作食品、食品工业和草药产品，也用作观赏品、动物饲料和其他商业领域。黄秋葵富含生物活性化合物，如类黄酮、多糖、多酚、咖啡因和果胶。对黄秋葵不同器官中总黄酮和多糖含量进行了测定和比较。利用转录组测序技术探索黄秋葵活性成分的生物合成途径。对黄秋葵的五个器官（根、茎、叶、花和果实）进行转录组测序，使我们能够获得293，971个unigenes，其中232，490个被注释。基于京都基因和基因组百科全书（KEGG）途径分析，鉴定了与类黄酮生物合成途径或果糖和甘露糖代谢中涉及的酶相关的单基因。将所有转录数据集上传到序列读取档案（SRA）。总之，我们的综合分析提供了重要的信息，在分子水平上的黄酮和多糖的生物合成途径在黄秋葵。
Abelmoschus esculentus (okra or lady’s fingers) is a vegetable with high nutritional value, as well as having certain medicinal effects. It is widely used as food, in the food industry, and in herbal medicinal products, but also as an ornamental, in animal feed, and in other commercial sectors. Okra is rich in bioactive compounds, such as flavonoids, polysaccharides, polyphenols, caffeine, and pectin. In the present study, the concentrations of total flavonoids and polysaccharides in five organs of okra were determined and compared. Transcriptome sequencing was used to explore the biosynthesis pathways associated with the active constituents in okra. Transcriptome sequencing of five organs (roots, stem, leaves, flowers, and fruits) of okra enabled us to obtain 293,971 unigenes, of which 232,490 were annotated. Unigenes related to the enzymes involved in the flavonoid biosynthetic pathway or in fructose and mannose metabolism were identified, based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. All of the transcriptional datasets were uploaded to Sequence Read Archive (SRA). In summary, our comprehensive analysis provides important information at the molecular level about the flavonoid and polysaccharide biosynthesis pathways in okra.
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DOI：
10.1073/pnas.94.5.2085
发表时间：
1997-03-04
影响因子：
11.1
作者：
Bonin, CP;Potter, I;Reiter, WD
通讯作者：
Reiter, WD
影响因子：
4.9
作者：
Badejo, Adebanjo A.;Eltelib, Hani A.;Esaka, Muneharu
通讯作者：
Esaka, Muneharu
影响因子：
4.4
作者：
Liang C;Liu X;Yiu SM;Lim BL
通讯作者：
Lim BL
影响因子：
5.1
作者：
Hemleben, V;Dressel, A;Austin, MB
通讯作者：
Austin, MB
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M