Development of an indirect competitive ELISA for the detection of acenaphthene and pyrene
Development of an indirect competitive ELISA for the detection of acenaphthene and pyrene
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开发用于检测苊和芘的间接竞争 ELISA
DOI：
10.1080/09540105.2017.1313201
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发表时间：
2017-04
影响因子：
3
通讯作者：
Zhou Yu
中科院分区：
文献类型：
--
作者：
Qiao Bin;Li Yansong;Meng Xingyu;Sun Yu;Hu Pan;Lu Shiying;Ren Honglin;Liu Zengshan;Zhou Yu
摘要多环芳烃（PAHs）具有致突变性和致癌性。苊和芘是美国环境保护署列为水环境优先污染物的16种多环芳烃之一。所报道的仪器方法和免疫测定法不能满足对苊和芘的简单和灵敏检测的需要。本研究制备了与苊和芘具有高亲和力的单克隆抗体，并建立了间接竞争酶联免疫吸附试验（ELISA），用于检测水样中的苊和芘。测定的线性范围为3.53 - 41.98 ng mL−1。 灵敏度为12.17 ng mL−1，比已报道的ELISA灵敏度高。 3种水样中苊和芘的平均回收率分别为99.08%和98.45%。该方法可用于水样中苊和芘的灵敏检测。
ABSTRACT Polycyclic aromatic hydrocarbons (PAHs) have mutagenic and carcinogenic properties. Acenaphthene and pyrene were members of 16 PAHs which were listed as the priority pollutants in water environment by US Environmental Protection Agency. The reported instrumental methods and immunoassays did not meet the need for simple and sensitive detection of acenaphthene and pyrene. In this study, a monoclonal antiobody having high affinities with acenaphthene and pyrene was produced and an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for sensitive detection of acenaphthene and pyrene in water sample. The linear range of the assay was between 3.53 and 41.98 ng mL−1. The sensitivity was 12.17 ng mL−1 which was more sensitive than those of reported ELISAs. The average recovery of acenaphthene and pyrene from three kinds of water samples was 99.08% and 98.45, respectively. The developed ELISA could be used for sensitive detection of acenaphthene and pyrene in water samples.
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影响因子：
3.1
作者：
Chen, Yanni;Kong, Dezhao;Xu, Chuanlai
通讯作者：
Xu, Chuanlai
影响因子：
12.6
作者：
S. Boujday;S. Nasri;M. Salmain;C. Pradier
通讯作者：
S. Boujday;S. Nasri;M. Salmain;C. Pradier
影响因子：
6.1
作者：
Qin Zhao;F. Wei;Yan-Bo Luo;Jun Ding;Neng Xiao;Yuqi Feng
通讯作者：
Qin Zhao;F. Wei;Yan-Bo Luo;Jun Ding;Neng Xiao;Yuqi Feng
影响因子：
2.2
作者：
Y. Yi;Zhu-ming Wang;Min Li;Keyin Zhu;Guoqing Ying
通讯作者：
Y. Yi;Zhu-ming Wang;Min Li;Keyin Zhu;Guoqing Ying
影响因子：
0.7
作者：
Andrew N. Rowan
通讯作者：
Andrew N. Rowan