ELO2 Participates in the Regulation of Osmotic Stress Response by Modulating Nitric Oxide Accumulation in Arabidopsis.
ELO2 Participates in the Regulation of Osmotic Stress Response by Modulating Nitric Oxide Accumulation in Arabidopsis.
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DOI：
10.3389/fpls.2022.924064
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
ELO家族参与合成超长链脂肪酸（VLCFA），VLCFA在植物发育、蛋白质运输和抗病性中起关键作用，但植物ELO家族的生理功能在很大程度上是未知的。此外，虽然一氧化氮合酶（NOS）样活性通过调节一氧化氮（NO）的积累在各种植物环境响应中起作用，但是在这些不同的胁迫响应中如何调节NOS样活性仍然是模糊的。在这里，我们报告酵母突变体Δ elo 3在H2 O2触发的细胞凋亡中存在缺陷，NOS样活性和NO积累减少，而其拟南芥同源基因ELO 2（ELO HOMOLOG 2）可以补充Δ elo 3中的此类缺陷。该基因的表达增强，并在植物渗透胁迫响应所需的，因为T-DNA插入突变体elo2比野生型植物对胁迫更敏感，和Elo2的表达可以挽救elo2的敏感性表型。此外，渗透胁迫促进的NOS样活性和NO积累显着抑制elo2，而外源应用NO供体可以拯救这种敏感性elo2的发芽率，鲜重，叶绿素含量和离子泄漏。此外，在渗透胁迫下，elo2与野生型相比，胁迫响应基因表达、脯氨酸积累和过氧化氢酶活性也受到抑制。综上所述，我们的研究确定了NOS2是参与植物渗透胁迫反应的关键因子，并揭示了其在调节NOS活性和NO积累中的作用。
The ELO family is involved in synthesizing very-long-chain fatty acids (VLCFAs) and VLCFAs play a crucial role in plant development, protein transport, and disease resistance, but the physiological function of the plant ELO family is largely unknown. Further, while nitric oxide synthase (NOS)-like activity acts in various plant environmental responses by modulating nitric oxide (NO) accumulation, how the NOS-like activity is regulated in such different stress responses remains misty. Here, we report that the yeast mutant Δelo3 is defective in H2O2-triggered cell apoptosis with decreased NOS-like activity and NO accumulation, while its Arabidopsis homologous gene ELO2 (ELO HOMOLOG 2) could complement such defects in Δelo3. The expression of this gene is enhanced and required in plant osmotic stress response because the T-DNA insertion mutant elo2 is more sensitive to the stress than wild-type plants, and ELO2 expression could rescue the sensitivity phenotype of elo2. In addition, osmotic stress-promoted NOS-like activity and NO accumulation are significantly repressed in elo2, while exogenous application of NO donors can rescue this sensitivity of elo2 in terms of germination rate, fresh weight, chlorophyll content, and ion leakage. Furthermore, stress-responsive gene expression, proline accumulation, and catalase activity are also repressed in elo2 compared with the wild type under osmotic stress. In conclusion, our study identifies ELO2 as a pivotal factor involved in plant osmotic stress response and reveals its role in regulating NOS-like activity and NO accumulation.
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DOI：
10.3390/antiox10040611
发表时间：
2021-04-16
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
Hasanuzzaman M;Inafuku M;Nahar K;Fujita M;Oku H
通讯作者：
Oku H
影响因子：
3.7
作者：
Cai W;Liu W;Wang WS;Fu ZW;Han TT;Lu YT
通讯作者：
Lu YT
影响因子：
6.9
作者：
Castillo MC;Coego A;Costa-Broseta Á;León J
通讯作者：
León J
影响因子：
5.2
作者：
Arasimowicz-Jelonek, M.;Floryszak-Wieczorek, J.;Kubis, J.
通讯作者：
Kubis, J.
影响因子：
6
作者：
Batsale M;Bahammou D;Fouillen L;Mongrand S;Joubès J;Domergue F
通讯作者：
Domergue F