Overexpression of Rat Neurons Nitric Oxide Synthase in Rice Enhances Drought and Salt Tolerance.
Overexpression of Rat Neurons Nitric Oxide Synthase in Rice Enhances Drought and Salt Tolerance.
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DOI：
10.1371/journal.pone.0131599
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Lu YT
中科院分区：
文献类型：
--
作者：
Cai W;Liu W;Wang WS;Fu ZW;Han TT;Lu YT
一氧化氮（NO）已被证明在拟南芥突变体对生物和非生物胁迫的反应中发挥重要作用，内源NO水平较高或较低。一氧化氮供体或清除剂的外源应用也表明一氧化氮在植物抵御环境胁迫中的重要作用。在干旱和高盐条件下，水稻植株一氧化氮合酶（NOS）活性和一氧化氮水平均有所升高。水稻中过表达大鼠神经元NO合成酶（nNOS）增加了NOS活性和NO积累，从而提高了转基因植株对干旱和盐胁迫的耐受性。与野生稻相比，过表达nnos的植株在干旱和盐胁迫下表现出更强的持水能力、更高的脯氨酸积累、更少的脂质过氧化和更少的电解质泄漏。此外，由于OsCATA、OsCATB和OsPOX1的上调，nnos过表达植株积累的H2O2较少。与此一致的是，转基因水稻的CAT和POX活性高于野生型。此外，干旱和高盐胁迫条件下，nnos过表达植株中OsDREB2A、OsDREB2B、OsSNAC1、OsSNAC2、OsLEA3和OsRD29A 6个胁迫响应基因的表达量均高于野生型。综上所述，我们的研究结果表明，nNOS过表达抑制应激增强的电解质泄漏、脂质过氧化和H2O2积累，促进脯氨酸积累和应激响应基因的表达，从而促进对干旱和盐胁迫的耐受性提高。
Nitric oxide (NO) has been shown to play an important role in the plant response to biotic and abiotic stresses in Arabidopsis mutants with lower or higher levels of endogenous NO. The exogenous application of NO donors or scavengers has also suggested an important role for NO in plant defense against environmental stress. In this study, rice plants under drought and high salinity conditions showed increased nitric oxide synthase (NOS) activity and NO levels. Overexpression of rat neuronal NO synthase (nNOS) in rice increased both NOS activity and NO accumulation, resulting in improved tolerance of the transgenic plants to both drought and salt stresses. nNOS-overexpressing plants exhibited stronger water-holding capability, higher proline accumulation, less lipid peroxidation and reduced electrolyte leakage under drought and salt conditions than wild rice. Moreover, nNOS-overexpressing plants accumulated less H2O2, due to the observed up-regulation of OsCATA, OsCATB and OsPOX1. In agreement, the activities of CAT and POX were higher in transgenic rice than wild type. Additionally, the expression of six tested stress-responsive genes including OsDREB2A, OsDREB2B, OsSNAC1, OsSNAC2, OsLEA3 and OsRD29A, in nNOS-overexpressing plants was higher than that in the wild type under drought and high salinity conditions. Taken together, our results suggest that nNOS overexpression suppresses the stress-enhanced electrolyte leakage, lipid peroxidation and H2O2 accumulation, and promotes proline accumulation and the expression of stress-responsive genes under stress conditions, thereby promoting increased tolerance to drought and salt stresses.
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