Laser-Induced Graphene Decorated with Platinum Nanoparticles for Electrochemical Analysis of Saliva
Laser-Induced Graphene Decorated with Platinum Nanoparticles for Electrochemical Analysis of Saliva
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用铂纳米粒子修饰的激光诱导石墨烯用于唾液的电化学分析
DOI：
10.1021/acsanm.3c03786
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发表时间：
2023
影响因子：
5.9
通讯作者：
Gomes, Carmen L.
中科院分区：
文献类型：
--
作者：
Hjort, Robert G.;Pola, Cícero C.;Soares, Raquel R.;Opare-Addo, Jemima;Smith, Emily A.;Claussen, Jonathan C.;Gomes, Carmen L.
唾液传感器在医学诊断和运动医学中变得越来越重要，这是由于在流体中内源性地发现了过多的生物医学相关分析物以及感测协议的固有非侵入性。唾液乳酸盐和钾已被证明是监测疲劳，水合作用和整体健康的关键指标，经常监测对于预防性治疗策略至关重要。结合唾液拭子采样的低成本试纸条传感器可以实现这种频繁的监测，为此，我们使用激光诱导石墨烯（LIG）开发了电流型乳酸盐和库仑钾试纸条传感器，这是一种用于石墨烯传感器开发的低成本可扩展制造方法。为了提高传感器的灵敏度，铂纳米颗粒（nPt）沉积在LIG表面上，通过使用一个简单的化学沉积方法。随后，氧化还原介体与酶乳酸氧化酶用于乳酸传感，而基于聚合物的离子选择性膜用于钾传感。乳酸传感器的灵敏度为33.3 ± 0.9 μA mM-1cm-2，检测限（LOD）为0.10 ± 0.06 mM;钾传感器的灵敏度为168.2 μC dec-1，信噪比（S/N）为193.3 ± 56.2。两种传感器都能够在生理相关范围内选择性地感测真实的合并唾液样本。这样的传感结果证明了nPt-LIG用于个性化健康和运动表现监测的即时生物传感器的潜力。
Saliva-based sensors are becoming increasingly important in medical diagnostics and sports medicine due to the plethora of biomedically relevant analytes found endogenously within the fluid and to the inherently noninvasive nature of the sensing protocols. Salivary lactate and potassium have been shown to be key indicators for monitoring fatigue, hydration, and overall health, and frequent monitoring is crucial for preventative treatment strategies. Low-cost test strip sensors combined with saliva swab sampling could enable such frequent monitoring, and to this end, we developed amperometric lactate and coulometric potassium test strip sensors using laser-induced graphene (LIG), a low-cost scalable fabrication method for graphene sensor development. To increase the sensitivity of the sensors, platinum nanoparticles (nPt) were deposited on the LIG surface by using a facile electroless deposition method. Subsequently, a redox mediator with the enzyme lactate oxidase was used for lactate sensing, while a polymer-based ion-selective membrane was used for potassium sensing. The lactate biosensor displayed a sensitivity of 33.3 ± 0.9 μA mM–1cm–2and a limit of detection (LOD) of 0.10 ± 0.06 mM, while the potassium sensor exhibited a sensitivity of 168.2 μC dec–1and a signal-to-noise ratio (S/N) of 193.3 ± 56.2. Both sensors were capable of selectively sensing within the physiologically relevant range with real pooled saliva samples. Such sensing results demonstrate the potential of nPt-LIG for point-of-care biosensors for personalized health and athletic performance monitoring.
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影响因子：
6.1
作者：
Madden, Julia;Vaughan, Eoghan;Teixeira, Sofia Rodrigues
通讯作者：
Teixeira, Sofia Rodrigues
影响因子：
5.7
作者：
Robert G. Hjort;Raquel R. A. Soares;Jingzhe Li;Dapeng Jing;Lindsey M. Hartfiel;Bolin Chen;B. Van Belle;M. Soupir;Emily A. Smith;E. McLamore;J. Claussen;C. Gomes
通讯作者：
Robert G. Hjort;Raquel R. A. Soares;Jingzhe Li;Dapeng Jing;Lindsey M. Hartfiel;Bolin Chen;B. Van Belle;M. Soupir;Emily A. Smith;E. McLamore;J. Claussen;C. Gomes
影响因子：
4.9
作者：
Bryce, MR;Devonport, W;Wang, CS
通讯作者：
Wang, CS
影响因子：
12.6
作者：
Lim, Hyo-Ryoung;Lee, Soon Min;Yeo, Woon-Hong
通讯作者：
Yeo, Woon-Hong
影响因子：
--
作者：
Bagalad BS;Mohankumar KP;Madhushankari GS;Donoghue M;Kuberappa PH
通讯作者：
Kuberappa PH