Early changes of microRNAs expression in the dorsal root ganglia following rat sciatic nerve transection
Early changes of microRNAs expression in the dorsal root ganglia following rat sciatic nerve transection
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大鼠坐骨神经横断后背根神经节 microRNA 表达的早期变化
DOI：
10.1016/j.neulet.2011.02.064
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发表时间：
2011-04
影响因子：
2.5
通讯作者：
Zhou, Songlin
中科院分区：
文献类型：
--
作者：
Qian, Tianmei;Gu, Xiaosong;Yu, Bin;Ding, Fei;Wang, Yongjun;Yao, Dengbing;Zhou, Songlin
microRNA（miRNAs）是一类新型的非编码小RNA，在转录后水平调控基因表达。在这里，我们报告的早期变化的miRNAs表达大鼠坐骨神经损伤后，使用微阵列分析。我们收集背根神经节（DRG）组织，并确定了19个miRNAs，在坐骨神经切断后的四个早期时间点显示出显着变化。随后，通过实时定量逆转录酶聚合酶链反应（qRT-PCR）验证miR-188和miR-500微阵列结果。生物信息学分析表明，这些miRNAs的潜在作用靶点涉及细胞内信号级联、信号转导调节、细胞过程调控以及对cAMP的反应，在坐骨神经损伤早期动员神经突起生长和促进再生的内在能力中发挥重要作用。我们的研究结果表明，miRNAs的异常表达可能有助于阐明神经再生的分子机制，miRNAs是可能增强内在再生能力的治疗干预的潜在靶点。
MicroRNAs (miRNAs) are a novel class of small non-coding RNAs that regulate gene expression at the post-transcriptional level. Here we report early alterations of miRNAs expression following rat sciatic nerve injury using microarray analysis. We harvested dorsal root ganglia (DRG) tissues and identified 19 miRNAs that showed significant changes at four early time points after sciatic nerve transection. Subsequently, miR-188 and miR-500 microarray results were verified by real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The bioinformatics analysis indicated that the potential targets for these miRNAs were involved in the intracellular signaling cascade, the regulation of signal transduction, the regulation of cellular process and the response to cAMP that were known to play important roles in mobilizing the inherent capacity for neurite outgrowth and promoting regeneration during the early phase of sciatic nerve injury. Our results show that abnormal expression of miRNAs may contribute to illustrate the molecular mechanisms of nerve regeneration and miRNAs are potential targets for therapeutic interventions that may enhance intrinsic regenerative ability.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
16.2
作者：
Neumann, S;Bradke, F;Basbaum, AI
通讯作者：
Basbaum, AI
影响因子：
5.3
作者：
D'Antonio, Maurizio;Droggiti, Anna;Jessen, Kristjan R.
通讯作者：
Jessen, Kristjan R.
创伤性脊髓损伤后的microRNA表达改变。
影响因子：
5.3
作者：
Liu, Nai-Kui;Wang, Xiao-Fei;Lu, Qing-Bo;Xu, Xiao-Ming
通讯作者：
Xu, Xiao-Ming
影响因子：
2.5
作者：
Nelson PT;Wilfred BR
通讯作者：
Wilfred BR