Immunological and metabolomic impacts of administration of Cry1Ab protein and MON 810 maize in mouse.
Immunological and metabolomic impacts of administration of Cry1Ab protein and MON 810 maize in mouse.
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DOI：
10.1371/journal.pone.0016346
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发表时间：
2011-01-27
期刊：
影响因子：
3.7
通讯作者：
Wal JM
中科院分区：
文献类型：
--
作者：
Adel-Patient K;Guimaraes VD;Paris A;Drumare MF;Ah-Leung S;Lamourette P;Nevers MC;Canlet C;Molina J;Bernard H;Créminon C;Wal JM
我们研究了Cry 1Ab给药对小鼠的免疫学和代谢组学影响，无论是作为纯化蛋白还是作为表达Cry 1Ab的转基因（GM）MON 810玉米。BALB/cJ小鼠胃内（i.g.）或腹膜内（i. p.）分析了纯化的Cry 1Ab的施用，并与由各种免疫原性和过敏效力的蛋白质诱导的那些进行比较。玉米天然过敏原的表达模式的遗传修饰可能产生的意外影响进行了研究，使用IgE-免疫印迹和血清从玉米过敏患者。将小鼠实验致敏（i.g.或i. p.途径）与来自转基因或非转基因玉米的蛋白质提取物接触，然后分析抗玉米蛋白和抗Cry 1Ab诱导的免疫应答。平行地，对通过i.g.路线在i.g.不同蛋白质的管理。使用腹膜内途径，用已知的变应原性蛋白质观察到明显的Th 2应答，而用未知变应原性的免疫原性蛋白质和Cry 1Ab观察到混合的Th 1/Th 2免疫应答。这反映了BALB/c Th 2偏向小鼠品系中的蛋白免疫原性，而不是变应原性。MON 810与其非转基因对照品之间的天然玉米过敏原谱无差异。在接受MON 810处理的小鼠中，针对玉米蛋白的免疫应答在数量上与非转基因对应物相当，并且在接受MON 810的小鼠中未检测到抗Cry 1Ab特异性免疫应答。代谢组学研究表明，轻微的“品种”的影响，这代表了不到1%的初始代谢信息。我们的研究结果证实了纯化的Cry 1Ab的免疫原性，没有证据表明其具有致敏性。免疫学和代谢组学研究显示，i.g.因此，M0 N810的施用与其非GM对应物相比，没有观察到遗传修饰对免疫应答的显著的非预期影响。
We have investigated the immunological and metabolomic impacts of Cry1Ab administration to mice, either as a purified protein or as the Cry1Ab-expressing genetically modified (GM) MON810 maize. Humoral and cellular specific immune responses induced in BALB/cJ mice after intra-gastric (i.g.) or intra-peritoneal (i.p.) administration of purified Cry1Ab were analyzed and compared with those induced by proteins of various immunogenic and allergic potencies. Possible unintended effects of the genetic modification on the pattern of expression of maize natural allergens were studied using IgE-immunoblot and sera from maize-allergic patients. Mice were experimentally sensitized (i.g. or i.p. route) with protein extracts from GM or non-GM maize, and then anti-maize proteins and anti-Cry1Ab–induced immune responses were analyzed. In parallel, longitudinal metabolomic studies were performed on the urine of mice treated via the i.g. route. Weak immune responses were observed after i.g. administration of the different proteins. Using the i.p. route, a clear Th2 response was observed with the known allergenic proteins, whereas a mixed Th1/Th2 immune response was observed with immunogenic protein not known to be allergenic and with Cry1Ab. This then reflects protein immunogenicity in the BALB/c Th2-biased mouse strain rather than allergenicity. No difference in natural maize allergen profiles was evidenced between MON810 and its non-GM comparator. Immune responses against maize proteins were quantitatively equivalent in mice treated with MON810 vs the non-GM counterpart and no anti-Cry1Ab–specific immune response was detected in mice that received MON810. Metabolomic studies showed a slight “cultivar” effect, which represented less than 1% of the initial metabolic information. Our results confirm the immunogenicity of purified Cry1Ab without evidence of allergenic potential. Immunological and metabolomic studies revealed slight differences in mouse metabolic profiles after i.g. administration of MON810 vs its non-GM counterpart, but no significant unintended effect of the genetic modification on immune responses was seen.
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影响因子：
6.1
作者：
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通讯作者：
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