Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast.
Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast.
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DOI：
10.1101/gr.225615.117
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发表时间：
2018-03
期刊：
影响因子：
7
通讯作者：
Beggs JD
中科院分区：
文献类型：
--
作者：
Aslanzadeh V;Huang Y;Sanguinetti G;Beggs JD
选择性剪接对改变转录速率的功能后果一直是哺乳动物细胞中深入研究的主题，但对剪接对改变酵母中转录速率的影响知之甚少。我们提出了几条线的证据表明，缓慢的RNA聚合酶II延长增加共转录剪接和剪接效率和更快的延长降低共转录剪接和剪接效率在芽殖酵母，这表明剪接是更有效的共转录时。此外，我们表明，改变RNA聚合酶II的延伸率在任何方向上损害剪接保真度，我们揭示，剪接保真度在很大程度上取决于内含子长度连同二级结构和剪接位点得分。这些作用对于高表达的核糖体蛋白编码转录物明显更强。我们建议，RNA聚合酶II的转录被调整，以优化核糖体蛋白基因表达的效率和准确性，同时允许灵活的剪接位点选择与非核糖体蛋白转录。
The functional consequences of alternative splicing on altering the transcription rate have been the subject of intensive study in mammalian cells but less is known about effects of splicing on changing the transcription rate in yeast. We present several lines of evidence showing that slow RNA polymerase II elongation increases both cotranscriptional splicing and splicing efficiency and that faster elongation reduces cotranscriptional splicing and splicing efficiency in budding yeast, suggesting that splicing is more efficient when cotranscriptional. Moreover, we demonstrate that altering the RNA polymerase II elongation rate in either direction compromises splicing fidelity, and we reveal that splicing fidelity depends largely on intron length together with secondary structure and splice site score. These effects are notably stronger for the highly expressed ribosomal protein coding transcripts. We propose that transcription by RNA polymerase II is tuned to optimize the efficiency and accuracy of ribosomal protein gene expression, while allowing flexibility in splice site choice with the nonribosomal protein transcripts.
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影响因子：
14.9
作者：
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通讯作者：
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影响因子：
16
作者：
Dujardin, Gwendal;Lafaille, Celina;Kornblihtt, Alberto R.
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影响因子：
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作者：
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通讯作者：
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