Identification of small non-coding RNAs from mitochondria and chloroplasts.
Identification of small non-coding RNAs from mitochondria and chloroplasts.
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DOI：
10.1093/nar/gkl448
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发表时间：
2006
影响因子：
14.9
通讯作者：
Hüttenhofer A
中科院分区：
文献类型：
--
作者：
Lung B;Zemann A;Madej MJ;Schuelke M;Techritz S;Ruf S;Bock R;Hüttenhofer A
小的非蛋白编码rna （ncRNAs）已经在从细菌到人类的广泛生物中被发现。在真核生物中，到目前为止，对ncrna的系统搜索仅限于细胞的核室或细胞质室。除了trna或核糖体RNA外，细胞器中是否还存在稳定的小非编码RNA物种，目前尚不清楚。因此，我们从大小选择的哺乳动物线粒体RNA和植物叶绿体RNA中生成cDNA文库，并在这两种含dna的细胞器中寻找小的ncRNA物种。总的来说，我们已经在这两个细胞区室中确定了18个新的细胞器ncrna候选者，并通过northern blot分析或RNase A保护试验证实了其中6个的表达。大多数候选ncRNA基因映射到细胞器基因组的基因间区域。正如之前在细菌中发现的那样，我们也观察到可能靶向细胞器编码mrna的反义ncrna的例子。细菌被认为是当今叶绿体和线粒体的祖先。讨论了鉴定的ncrna的结构特征及其可能的细胞功能。我们的文库中没有大量不被细胞器基因组编码的小RNA物种，这表明RNA进入细胞器的意义非常有限，或者根本没有发生。
Small non-protein-coding RNAs (ncRNAs) have been identified in a wide spectrum of organisms ranging from bacteria to humans. In eukarya, systematic searches for ncRNAs have so far been restricted to the nuclear or cytosolic compartments of cells. Whether or not small stable non-coding RNA species also exist in cell organelles, in addition to tRNAs or ribosomal RNAs, is unknown. We have thus generated cDNA libraries from size-selected mammalian mitochondrial RNA and plant chloroplast RNA and searched for small ncRNA species in these two types of DNA-containing cell organelles. In total, we have identified 18 novel candidates for organellar ncRNAs in these two cellular compartments and confirmed expression of six of them by northern blot analysis or RNase A protection assays. Most candidate ncRNA genes map to intergenic regions of the organellar genomes. As found previously in bacteria, the presumptive ancestors of present-day chloroplasts and mitochondria, we also observed examples of antisense ncRNAs that potentially could target organelle-encoded mRNAs. The structural features of the identified ncRNAs as well as their possible cellular functions are discussed. The absence from our libraries of abundant small RNA species that are not encoded by the organellar genomes suggests that the import of RNAs into cell organelles is of very limited significance or does not occur at all.
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通过比较基因组分析鉴定蓝细菌非编码RNA。
影响因子：
12.3
作者：
Axmann, Ilka M;Kensche, Philip;Vogel, Jorg;Kohl, Stefan;Herzel, Hanspeter;Hess, Wolfgang R
通讯作者：
Hess, Wolfgang R
影响因子：
4
作者：
BENDICH, AJ
通讯作者：
BENDICH, AJ
影响因子：
4
作者：
GRAY, MW
通讯作者：
GRAY, MW
DOI：
10.1007/bf00332900
发表时间：
1976-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
ATCHISON, BA;WHITFELD, PR;BOTTOMLEY, W
通讯作者：
BOTTOMLEY, W
影响因子：
--
作者：
Brennicke, A;Klein, M;Unseld, M
通讯作者：
Unseld, M