The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis.
The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis.
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使用线虫秀丽隐杆线虫评估氟环唑暴露对精子发生的不利影响
DOI：
10.3390/ijerph13100993
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发表时间：
2016-10-08
影响因子：
--
通讯作者：
Yin L
中科院分区：
文献类型：
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作者：
Li Y;Zhang M;Li S;Lv R;Chen P;Liu R;Liang G;Yin L
越来越多的证据表明，接触环氧康唑会影响生殖功能，但很少有研究调查环氧康唑对精子发生的不利影响。用线虫线虫研究了环氧康唑对雄性线虫精子发生的影响，结果表明，环氧康唑作用48h后，雄性线虫的精子发生受到影响，精子细胞总数、有丝分裂细胞、减数分裂细胞和精子细胞数、精子细胞直径和横截面积减少，有丝分裂生殖细胞增殖受阻，精子过早进入减数分裂，精子激活受到抑制，但精子移植未见异常改变。此外，环氧康唑还激活了转化生长因子-β信号通路，提高了线虫DAF-1、DAF-3、DAF-4、DAF-5和DAF-7基因的表达水平。因此，我们认为环氧康唑通过激活转化生长因子β信号通路，导致精子发生障碍，从而导致男性生育力下降。
There is increasing evidence that epoxiconazole exposure can affect reproductive function, but few studies have investigated adverse effects on spermatogenesis. The nematode Caenorhabditis elegans (C. elegans) was used in our study to assess effects of epoxiconazole on spermatogenesis in male nematodes after 48 h of exposure to concentrations of 0.1, 1.0, or 10.0 μg/L. The results demonstrated that epoxiconazole exposure affected spermatogenesis, decreasing the number of total germ cells, mitotic cells, meiotic cells and spermatids, spermatid diameter, and cross-sectional area, and inducing mitotic germ cell proliferation arrest, premature entry into meiosis, and sperm activation inhibition; however, sperm transfer showed no abnormal changes. In addition, the results showed that epoxiconazole activated the transforming growth factor-β (TGFβ) signaling pathway and increased the expression levels of gene daf-1, daf-3, daf-4, daf-5 and daf-7 in nematodes. We therefore propose that epoxiconazole acts by activating the TGFβ signaling pathway, leading to the impairment of spermatogenesis and the consequent decline in male fertility.
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影响因子：
16
作者：
Gartner, A;Milstein, S;Hengartner, MO
通讯作者：
Hengartner, MO
影响因子：
21.3
作者：
Kubagawa, Homare M.;Watts, Jennifer L.;Miller, Michael A.
通讯作者：
Miller, Michael A.
影响因子：
3.8
作者：
Heusinkveld, Harm J.;Molendijk, Jeffrey;Westerink, Remco H. S.
通讯作者：
Westerink, Remco H. S.
DOI：
10.1080/03601234.2013.824224
发表时间：
2013-12-02
影响因子：
2
作者：
Holeckova, Beata;Sivikova, Katarina;Galdikova, Martina
通讯作者：
Galdikova, Martina
影响因子：
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作者：
通讯作者：
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