secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus.

secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus.
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secA、secD、secF、yajC 和 yidC 有助于溶藻弧菌的粘附调节

DOI:
10.1002/mbo3.551
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发表时间:
2018-04
期刊:
影响因子:
3.4
通讯作者:
Yan Q
Yan Q
中科院分区:
生物学3区
文献类型:
--
作者:
Guo L;Huang L;Su Y;Qin Y;Zhao L;Yan Q

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溶藻弧菌给水产养殖业造成了巨大的损失。粘附是溶藻弧菌的重要毒力因子。在这项研究中,溶藻弧菌粘附和II型分泌系统基因(secA,secD,secF,yajC和yidC)之间的关系是使用基因沉默,qRT-PCR和体外粘附试验来确定的。结果表明,瞬时基因沉默和稳定基因沉默后,目的基因的表达量和细菌粘附能力均显著降低,表明secA、secD、secF、yajC和yidC在溶藻弧菌的细菌粘附中起作用。secA、secD、secF、yajC和yidC的表达受温度、盐度、pH和饥饿的影响显著。结果表明,secA、secD、secF、yajC和yidC的表达对不同的环境因子敏感,而环境因子可通过secA、secD、secF、yajC和yidC的表达影响溶藻弧菌的粘附。
Vibrio alginolyticus caused great losses to aquaculture. Adhesion is an important virulence factor of V. alginolyticus. In this study, the relationship between V. alginolyticus adhesion and type II secretion system genes (secA, secD, secF, yajC, and yidC) was determined using gene silencing, qRT‐PCR and in vitro adhesion assay. The results showed that the expression of target genes and the bacterial adhesion exhibited significant decreases after transient gene silencing and stable gene silencing, which indicated that secA, secD, secF, yajC, and yidC played roles in the bacterial adhesion of V. alginolyticus. The expression of secA, secD, secF, yajC, and yidC were significantly influenced by temperature, salinity, pH and starvation. The results indicated that the expression of secA, secD, secF, yajC, and yidC were sensitive to different environmental factors, whereas environmental factors can affect V. alginolyticus adhesion via the expression of secA, secD, secF, yajC, and yidC.
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