Multicolor fluorescent semiconducting polymer dots with narrow emissions and high brightness.
Multicolor fluorescent semiconducting polymer dots with narrow emissions and high brightness.
复制标题
DOI：
10.1021/nn304376z
复制
发表时间：
2013-01-22
期刊：
影响因子：
17.1
通讯作者：
Chiu, Daniel T.
中科院分区：
文献类型：
--
作者：
Rong, Yu;Wu, Changfeng;Yu, Jiangbo;Zhang, Xuanjun;Ye, Fangmao;Zeigler, Maxwell;Gallina, Maria Elena;Wu, I-Che;Zhang, Yong;Chan, Yang-Hsiang;Sun, Wei;Uvdal, Kajsa;Chiu, Daniel T.
荧光半导体聚合物点（Pdot）由于其作为荧光探针的上级特性，如高的荧光亮度、快的辐射速率和优异的光稳定性，引起了人们极大的兴趣。然而，目前可用的Pdot通常表现出宽的发射光谱，这显著限制了它们在涉及多重检测的许多生物应用中的有用性。在这里，我们描述了基于不同的硼-二吡咯亚甲基（BODIPY）单元的窄发射Pdot的设计和开发。合成了在多个波长下发射的含BODIPY的半导体聚合物，并将其用作制备Pdot的前体，其中颗粒内能量转移导致高度明亮、窄的发射。所得聚合物量子点的发光半峰全宽（FWHM）为40 ~ 55 nm，比常规半导体聚合物量子点的发光半峰全宽窄1.5~2倍。在相同的激光激发条件下，BODIPY 520 Pdot比商业Qdot 525亮约一个数量级。荧光成像和流式细胞术实验表明，这些明亮的Pdot的窄发射是有希望的多重生物检测。
Fluorescent semiconducting polymer dots (Pdots) have attracted great interest because of their superior characteristics as fluorescent probes, such as high fluorescence brightness, fast radiative rates, and excellent photostability. However, currently available Pdots generally exhibit broad emission spectra, which significantly limit their usefulness in many biological applications involving multiplex detections. Here, we describe the design and development of multicolor narrow emissive Pdots based on different boron-dipyrromethene (BODIPY) units. BODIPY-containing semiconducting polymers emitting at multiple wavelengths were synthesized and used as precursors for preparing the Pdots, where intra-particle energy transfer led to highly bright, narrow emissions. The emission full-width at half maximum (FWHM) of the resulting Pdots varies from 40 nm to 55 nm, which is 1.5~2 times narrower than those of conventional semiconducting polymer dots. BODIPY520 Pdots was about an order of magnitude brighter than commercial Qdot 525 under identical laser excitation conditions. Fluorescence imaging and flow cytometry experiments indicate the narrow emissions from these bright Pdots are promising for multiplexed biological detections.
登录
查看更多内容
影响因子：
5.5
作者：
Nagai, Atsushi;Chujo, Yoshiki
通讯作者：
Chujo, Yoshiki
影响因子：
5.5
作者：
Alemdaroglu, Fikri E.;Alexander, Seth C.;Herrmann, Andreas
通讯作者：
Herrmann, Andreas
影响因子：
2.6
作者：
Kraszkiewicz, L;Sosnowski, M;Skulski, L
通讯作者：
Skulski, L
DOI：
10.1039/c2cc16486h
发表时间：
2012-02-07
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Ye F;Wu C;Jin Y;Wang M;Chan YH;Yu J;Sun W;Hayden S;Chiu DT
通讯作者：
Chiu DT
影响因子：
56.9
作者：
Bruchez, M;Moronne, M;Alivisatos, AP
通讯作者：
Alivisatos, AP