Linkage between C-reactive protein and triglyceride-rich lipoprotein metabolism.
Linkage between C-reactive protein and triglyceride-rich lipoprotein metabolism.
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DOI：
10.1016/j.metabol.2012.08.008
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发表时间：
2013-03
影响因子：
9.8
通讯作者：
Schaefer, Ernst J.
中科院分区：
文献类型：
--
作者：
Thongtang, Nuntakorn;Diffenderfer, Margaret R.;Ooi, Esther M. M.;Asztalos, Bela F.;Dolnikowski, Gregory G.;Lamon-Fava, Stefania;Schaefer, Ernst J.
炎症在动脉粥样硬化中起重要作用。C反应蛋白（CRP）水平升高与心血管疾病的风险增加有关。我们的目的是研究CRP代谢，并确定其与脂蛋白代谢的关系，用稳定同位素方法。8例合并高脂血症的受试者接受了15小时的氘代亮氨酸灌注。通过亲和色谱法从血浆密度大于1.21g/ml的组分中纯化CRP。通过连续超离心分离脂蛋白组分。同位素富集用气相色谱/质谱法测定。受试者的平均LDL-C水平为147.5 mg/dl，平均CRP水平为3.4 mg/ l。平均CRP生成率（PR）为0.050±0.012mg/kg/d，平均CRP部分分解率（FCR）为0.343±0.056池/d（停留时间2.92天）。CRP池大小（PS）与生产显著相关（r=0.93; p<0.001），但与FCR无关。CRP PS也与体重指数相关（r=0.79; p=0.02）。CRP FCR和TRL apoB-100 FCR之间存在显著相关性（r=0.74，p=0.04），CRP PS和TRL apoB-48 FCR之间也存在显著相关性（r=-0.90，p=0.002），表明CRP和TRL代谢之间存在关联。血浆CRP水平的主要决定因素是CRP产生率。此外，CRP代谢与TRL apoB-100和apoB-48催化剂之间存在显著联系。
Inflammation plays an important role in atherosclerosis. Elevated C-reactive protein (CRP) levels are associated with a greater risk of cardiovascular disease. Our goal was to study CRP metabolism, and to determine its relationship with lipoprotein metabolism using stable isotope methodology. Eight subjects with combined hyperlipidemia underwent a 15-h primed-constant infusion with deuterated leucine. CRP was purified from the plasma density fraction greater than 1.21g/ml by affinity chromatography. Lipoprotein fractions were separated by sequential ultracentrifugation. Isotope enrichment was determined by gas chromatography/mass spectrometry. The subjects had mean LDL-C levels of 147.5mg/dl and mean CRP levels of 3.4mg/ l. The mean CRP production rate (PR) was 0.050±0.012mg/kg/day and the mean CRP fractional catabolic rate (FCR) was 0.343±0.056 pools/day (residence time 2.92days). CRP pool size (PS) was significantly related to production (r=0.93; p<0.001), but not FCR. CRP PS was also related to body mass index (r=0.79; p=0.02). There was a significant association between CRP FCR and TRL apoB-100 FCR (r=0.74, p=0.04), as well as between CRP PS and TRL apoB-48 FCR (r=-0.90, p=0.002), indicating linkage between CRP and TRL metabolism. The main determinant of plasma CRP levels was CRP production rate. Moreover a significant linkage between CRP metabolism and both TRL apoB-100 and apoB-48 catabolism was noted.
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影响因子：
37.8
作者：
Pearson, TA;Mensah, GA;Vinicor, F
通讯作者：
Vinicor, F
影响因子：
15.9
作者：
Pepys, MB;Hirschfield, GM
通讯作者：
Hirschfield, GM
影响因子：
5.8
作者：
Maffeis, Claudio;Manfredi, Riccardo;Bonadonna, Riccardo C.
通讯作者：
Bonadonna, Riccardo C.
影响因子：
--
作者：
COBELLI, C;TOFFOLO, G;NOSADINI, R
通讯作者：
NOSADINI, R
影响因子：
6.5
作者：
Lamon-Fava, Stefania;Diffenderfer, Margaret R.;Schaefer, Ernst J.
通讯作者：
Schaefer, Ernst J.