sanN encoding a dehydrogenase is essential for Nikkomycin biosynthesis in Streptomyces ansochromogenes.
sanN encoding a dehydrogenase is essential for Nikkomycin biosynthesis in Streptomyces ansochromogenes.
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编码脱氢酶的 sanN 对于产色链霉菌中尼可霉素的生物合成至关重要。
DOI：
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发表时间：
2008-03
影响因子：
2.8
通讯作者：
中科院分区：
文献类型：
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作者：
尼可霉素是一组肽基核苷抗生素，具有有效的杀菌、杀虫和杀螨活性。 sanN was cloned from the partial genomic library of Streptomyces ansochromogenes 7100. Gene disruption and complementation analysis demonstrated that sanN is essential for nikkomycin biosynthesis in S. ansochromogenes. Primer extension assay indicated that sanN is transcribed from two promoters (sanN-P1 and sanN-P2), and sanN-P2 plays a more important role in nikkomycin biosynthesis. Purified recombinant SanN acts as a dehydrogenase to convert benzoate-CoA to benzaldehyde in a random-order mechanism in vitro, with respective Kcat/Km values of 3.8 mM-1s-1 and 12.0 mM-1s-1 toward benzoate-CoA and NADH, suggesting that SanN catalyzes the formation of picolinaldehyde during biosynthesis of nikkomycin X and Z components in the wild-type stain. These data would facilitate us to understand the biosynthetic pathway of nikkomycins and to consider the combinatorial synthesis of novel antibiotic derivatives.
Nikkomycins are a group of peptidyl nucleoside antibiotics with potent fungicidal, insecticidal, and acaricidal activities. sanN was cloned from the partial genomic library of Streptomyces ansochromogenes 7100. Gene disruption and complementation analysis demonstrated that sanN is essential for nikkomycin biosynthesis in S. ansochromogenes. Primer extension assay indicated that sanN is transcribed from two promoters (sanN-P1 and sanN-P2), and sanN-P2 plays a more important role in nikkomycin biosynthesis. Purified recombinant SanN acts as a dehydrogenase to convert benzoate-CoA to benzaldehyde in a random-order mechanism in vitro, with respective Kcat/Km values of 3.8 mM-1s-1 and 12.0 mM-1s-1 toward benzoate-CoA and NADH, suggesting that SanN catalyzes the formation of picolinaldehyde during biosynthesis of nikkomycin X and Z components in the wild-type stain. These data would facilitate us to understand the biosynthetic pathway of nikkomycins and to consider the combinatorial synthesis of novel antibiotic derivatives.
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DOI：
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发表时间：
2000
期刊：
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影响因子：
--
作者：
T. Kieser
通讯作者：
T. Kieser
DOI：
10.1016/s0006-291x(88)80898-2
发表时间：
1988-10
影响因子：
3.1
作者：
P. Engel;A. Ullah
通讯作者：
P. Engel;A. Ullah
影响因子：
3.3
作者：
BORMANN, C;MATTERN, S;ZAHNER, H
通讯作者：
ZAHNER, H
DOI：
10.1007/bf00409681
发表时间：
2004
期刊：
Archiv für Mikrobiologie
影响因子：
--
作者：
W. Pache;H. Zähner
通讯作者：
W. Pache;H. Zähner
DOI：
10.1046/j.1432-1327.1998.2540347.x
发表时间：
1998-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Bruntner, C;Bormann, C
通讯作者：
Bormann, C