Inhibition of interleukin 2‐induced proliferation of cloned murine T cells by glucocorticoids. Possible involvement of an inhibitory protein
Inhibition of interleukin 2‐induced proliferation of cloned murine T cells by glucocorticoids. Possible involvement of an inhibitory protein
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糖皮质激素对白细胞介素 2 诱导的克隆鼠 T 细胞增殖的抑制可能与抑制蛋白有关。
DOI：
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发表时间：
1988
影响因子：
5.4
通讯作者：
A. López
中科院分区：
文献类型：
--
作者：
J. Redondo;M. Fresno;A. López
在两个细胞毒性T细胞(CTL)克隆中，研究了糖皮质激素抑制白介素2(IL2)诱导T细胞增殖的能力。地塞米松(Dx)可完全抑制IL-2缺乏的CTL细胞在加入IL-2前与激素孵育6h，或同时加入低浓度的IL-2。与Dx孵育6h后，IL2受体的数量和亲和力均未见明显下降。不同类固醇的药效顺序表明，这种抑制作用是通过与特定的糖皮质激素受体结合而实现的。这些激素的作用可能涉及一种抑制蛋白的合成(S)，因为在与Dx孵育期间，放线菌酮的存在阻止了DNA合成的抑制。此外，Dx处理的CTL上清液中含有一种非透析性因子，该因子可抑制IL2诱导的CTL克隆的DNA合成和细胞生长。阻断IL-2合成和IL-2受体的形成是糖皮质激素诱导免疫抑制的主要机制之一。我们的结果表明，这些激素也可能通过抑制IL-2活性而影响T细胞的增殖。
The ability of glucocorticoids to inhibit interleukin 2 (IL2)‐induced T cell proliferation in two cytotoxic T cell (CTL) clones has been studied. A complete inhibition of DNA synthesis by dexamethasone (Dx) could be observed when IL2‐depleted cultures of CTL were either incubated for 6 h with the hormone prior to the addition of IL2 or treated simultaneously with Dx and a low concentration of IL2. No significant reduction in the number and affinity of IL2 receptors was seen after 6 h incubation with Dx. The order of potency observed with the different steroids indicated that this inhibitory effect was mediated through binding to a specific glucocorticoid receptor. The action of these hormones possibly involves the synthesis of an inhibitory protein(s), since the presence of cycloheximide during the incubation with Dx prevented the inhibition of DNA synthesis. Furthermore, supernatant from Dx‐treated CTL contained a nondialyzable factor which inhibited DNA synthesis and cell growth of CTL clones induced by IL2. Blocking of IL2 synthesis and IL2 receptor formation have been proposed as one of the major mechanisms of glucocorticoid‐induced immunosuppression. Our results indicate that these hormones may also affect T cell proliferation by inhibiting IL2 activity.
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影响因子：
56.9
作者：
CANTRELL, DA;SMITH, KA
通讯作者：
SMITH, KA
DOI：
10.1073/pnas.79.24.7659
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
EVANS, RM;BIRNBERG, NC;ROSENFELD, MG
通讯作者：
ROSENFELD, MG
影响因子：
15.9
作者：
GOODWIN, JS;ATLURU, D;LIANOS, EA
通讯作者：
LIANOS, EA
抗炎类固醇抑制 T 细胞介导的细胞毒性。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schleimer,RP;Jacques,A;Shin,HS;Lichtenstein,LM;Plaut,M
通讯作者：
Plaut,M
环孢素A和地塞米松对白细胞介素2受体基因表达的影响。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Reed,JC;Abidi,AH;Alpers,JD;Hoover,RG;Robb,RJ;Nowell,PC
通讯作者：
Nowell,PC