Spontaneous Depolarization-Induced Action Potentials of ON-Starburst Amacrine Cells during Cholinergic and Glutamatergic Retinal Waves.
Spontaneous Depolarization-Induced Action Potentials of ON-Starburst Amacrine Cells during Cholinergic and Glutamatergic Retinal Waves.
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DOI：
10.3390/cells9122574
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发表时间：
2020-12-01
期刊：
影响因子：
6
通讯作者：
Zhang DQ
中科院分区：
文献类型：
--
作者：
Yan RS;Yang XL;Zhong YM;Zhang DQ
发育中视网膜的相关自发活动（称为“视网膜波”）在改善视觉系统的神经回路中起着指导性作用。去极化（ON）和超极化（OFF）星爆无长突细胞（SAC）启动和传播胆碱能视网膜波。当胆碱能视网膜波停止时，SAC被认为是由ON双极细胞启动的胆碱能视网膜波驱动的。然而，胆碱能波和多巴胺能波在ON和OFF-SAC中的性质和功能仍然知之甚少。在目前的工作中，我们进行了全细胞膜片钳记录和Ca 2+成像从基因标记的ON和OFF-SACs在小鼠平板视网膜。我们发现，这两个SAC亚型表现出自发的节律性去极化在胆碱能和多巴胺能波。有趣的是，ON-SAC具有年龄依赖性的波诱导动作电位（AP），但OFF-SAC没有。同时钙成像和膜片钳记录表明，在胆碱能波，AP的ON-SAC似乎促进树突状细胞释放乙酰胆碱到相邻的ON-和OFF-SAC，这增强了他们的钙瞬变。这些结果促进了对发育中视网膜相关自发活动的细胞机制的理解。
Correlated spontaneous activity in the developing retina (termed “retinal waves”) plays an instructive role in refining neural circuits of the visual system. Depolarizing (ON) and hyperpolarizing (OFF) starburst amacrine cells (SACs) initiate and propagate cholinergic retinal waves. Where cholinergic retinal waves stop, SACs are thought to be driven by glutamatergic retinal waves initiated by ON-bipolar cells. However, the properties and function of cholinergic and glutamatergic waves in ON- and OFF-SACs still remain poorly understood. In the present work, we performed whole-cell patch-clamp recordings and Ca2+ imaging from genetically labeled ON- and OFF-SACs in mouse flat-mount retinas. We found that both SAC subtypes exhibited spontaneous rhythmic depolarization during cholinergic and glutamatergic waves. Interestingly, ON-SACs had wave-induced action potentials (APs) in an age-dependent manner, but OFF-SACs did not. Simultaneous Ca2+ imaging and patch-clamp recordings demonstrated that, during a cholinergic wave, APs of an ON-SAC appeared to promote the dendritic release of acetylcholine onto neighboring ON- and OFF-SACs, which enhances their Ca2+ transients. These results advance the understanding of the cellular mechanisms underlying correlated spontaneous activity in the developing retina.
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影响因子：
16.2
作者：
Akrouh A;Kerschensteiner D
通讯作者：
Kerschensteiner D
影响因子：
1.9
作者：
JENSEN, RJ
通讯作者：
JENSEN, RJ
DOI：
10.1002/cne.22200
发表时间：
2009-12-10
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Voinescu PE;Kay JN;Sanes JR
通讯作者：
Sanes JR
影响因子：
9.8
作者：
Hausselt SE;Euler T;Detwiler PB;Denk W
通讯作者：
Denk W
影响因子：
3.5
作者：
Kerschensteiner D
通讯作者：
Kerschensteiner D