Birthdays of retinal amacrine cell subtypes are systematically related to their molecular identity and soma position.
Birthdays of retinal amacrine cell subtypes are systematically related to their molecular identity and soma position.
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DOI：
10.1002/cne.22200
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发表时间：
2009-12-10
期刊：
影响因子：
--
通讯作者：
Sanes JR
中科院分区：
文献类型：
--
作者：
Voinescu PE;Kay JN;Sanes JR
哺乳动物的视网膜包含六种主要细胞类型，其中几种分子和形态学上不同的亚型，以了解在发育过程中如何产生亚型的多样性，我们专注于小鼠视网膜中的amacrine interneurons； 。基于神经递质和转录因子标记的子集。然后，我们使用溴脱氧尿苷（BRDU）标记来询问是否在不同时间出生（成为有丝分裂的），就像层层指定性的层层在甘氨酸链氨酸蛋白酶的平均生成2-3天，Gabagagic amacrine的生成。不同的时间。然而，到他们的目的地。适合他们的生日，支持他们在诞生的时间和地点附近指定的想法。
The mammalian retina contains six major cell types, several of which are divided into multiple molecularly and morphologically distinct subtypes. To understand how subtype diversity arises during development, we focused on amacrine interneurons in the mouse retina; ~30 amacrine subtypes have been identified in mammals. We used antibody markers to identify the two main amacrine subsets – GABAergic and glycinergic – and further subdivided these groups into smaller subsets based on expression of neurotransmitter and transcription factor markers. We then used bromodeoxyuridine (BrdU) labeling to ask whether amacrine subsets are born (become postmitotic) at different times, as is the case for lamina-specified subsets of cortical projection neurons. We found that GABAergic amacrines are generated on average 2–3 days before glycinergic amacrines. Moreover, subsets of GABAergic amacrines are born at distinct times. We also found a strong correlation between amacrine cell birthday and soma position in the mature retina, another point of similarity with cortical projection neurons. This relationship raised the possibility that amacrine subtype identity is determined by signals that uncommitted cells receive after they migrate to their destinations. However, cells labeled with BrdU in vivo, then dissociated and allowed to develop in vitro, acquired the amacrine subtype-specific markers appropriate for their birthdays, supporting the idea that they become specified near the time and place of their birth. Together, our results suggest that the birthdays of amacrine cells independently specify their destinations and subtype identities.
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影响因子：
16.2
作者：
HOLT, CE;BERTSCH, TW;HARRIS, WA
通讯作者：
HARRIS, WA
DOI：
10.1073/pnas.90.22.10841
发表时间：
1993-11-15
影响因子：
11.1
作者：
GERRERO, MR;MCEVILLY, RJ;ROSENFELD, MG
通讯作者：
ROSENFELD, MG
影响因子：
2.5
作者：
Haverkamp, S;Wässle, H
通讯作者：
Wässle, H
影响因子：
2.5
作者：
Elshatory, Yasser;Deng, Min;Gan, Lin
通讯作者：
Gan, Lin
影响因子：
3.4
作者：
EVANS, JA;BATTELLE, BA
通讯作者：
BATTELLE, BA