Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests.
Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests.
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DOI：
10.1016/j.jviromet.2013.10.014
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发表时间：
2014-03
影响因子：
3.1
通讯作者：
Hohdatsu T
中科院分区：
文献类型：
--
作者：
Takano T;Ishihara Y;Matsuoka M;Yokota S;Matsuoka-Kobayashi Y;Doki T;Hohdatsu T
设计了三种类型的免疫层析检测（ICA）来检测抗猫冠状病毒（FCoV）抗体。重组FCoV核衣壳蛋白（rNP）在所有3种伊卡试剂盒（CJIgG/TNP、CJNP/TNP和CJNP/TPA）中用作偶联物或测试线。3种伊卡试剂盒均能检测抗FCoV抗体，但以rNP为检测线的2种伊卡试剂盒（CJIgG/TNP和CJNP/TNP）均观察到抗FCoV抗体阴性血浆样本与检测线的非特异性阳性反应。另一方面，使用伊卡试剂盒，以蛋白A印迹作为检测线（CJNP/TPA），可以在所有血浆、血清、全血和腹水样本中特异性检测抗FCoV抗体。此外，伊卡（CJNP/TPA）的特异性和敏感性与参考ELISA相当。预计未来将开发使用伊卡（CJNP/TPA）原理的其他冠状病毒和猫病毒感染的简单抗体检测方法。
Three types of immunochromatographic assays (ICAs) were designed to detect anti-feline coronavirus (FCoV) antibodies. Recombinant FCoV nucleocapsid protein (rNP) was used as a conjugate or test line in all 3 ICA kits (CJIgG/TNP, CJNP/TNP, and CJNP/TPA). All three ICA kits were capable of detecting anti-FCoV antibodies; however, non-specific positive reactions of anti-FCoV antibody-negative plasma samples with the test line were observed in 2 ICA kits (CJIgG/TNP and CJNP/TNP), in which rNP was used as the test line. On the other hand, the specific detection of anti-FCoV antibodies was possible in all plasma, serum, whole blood, and ascitic fluid samples using the ICA kit with protein A blotted as the test line (CJNP/TPA). In addition, the specificity and sensitivity of ICA (CJNP/TPA) were equivalent to those of the reference ELISA. The development of simple antibody test methods using the principle of ICA (CJNP/TPA) for other coronavirus and feline viral infections is expected in the future.
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影响因子：
--
作者：
Severance, Emily G.;Bossis, Ioannis;Viscidi, Raphael P.
通讯作者：
Viscidi, Raphael P.
影响因子：
11.8
作者：
Chang HW;Egberink HF;Halpin R;Spiro DJ;Rottier PJ
通讯作者：
Rottier PJ
影响因子：
1.7
作者：
Addie D;Belák S;Boucraut-Baralon C;Egberink H;Frymus T;Gruffydd-Jones T;Hartmann K;Hosie MJ;Lloret A;Lutz H;Marsilio F;Pennisi MG;Radford AD;Thiry E;Truyen U;Horzinek MC
通讯作者：
Horzinek MC
DOI：
10.1128/cdli.11.2.417-422.2004
发表时间：
2004-03-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
He, QG;Chong, KH;Kwang, J
通讯作者：
Kwang, J
影响因子：
3.1
作者：
Cui, Shangjin;Zhou, Shenghua;Oh, JinSik
通讯作者：
Oh, JinSik