In vivo cytokine response to experimental feline infectious peritonitis virus infection.
In vivo cytokine response to experimental feline infectious peritonitis virus infection.
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DOI：
10.1016/j.vetmic.2003.08.010
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发表时间：
2003-12-02
影响因子：
3.3
通讯作者：
Pedersen NC
中科院分区：
文献类型：
--
作者：
Dean GA;Olivry T;Stanton C;Pedersen NC
猫感染性腹膜炎病毒（FIPV）是一种冠状病毒，在猫炎，肉芽肿性感染和胸膜炎/腹膜炎的猫中引起零星的致命疾病在整个淋巴组织中，没有发现组织学病变在76％的中央组织（纵隔淋巴结，脾脏，肠系膜淋巴结）中发现，与27％的外围组织（宫颈淋巴结，宫颈淋巴结，股骨骨髓）也是所有组织阳性的外周淋巴结，宫颈淋巴结，宫颈淋巴结）。表现出的淋巴样耗竭。在受影响的组织中的表达更大，并且表现出TNF-α的源头从巨噬细胞转移到淋巴细胞，这些结果将FIPV复制，组织中的淋巴细胞部署和细胞因子转录和翻译的作用变化。在先前描述的FIPV诱导的淋巴细胞凋亡中。
Feline infectious peritonitis virus (FIPV) is a coronavirus that causes sporadic fatal disease in cats characterized by vasculitis, granulomatous inflammation and effusive pleuritis/peritonitis. Histologic changes in lymphoid tissues include lymphoid hyperplasia, lymphoid depletion, histiocytosis, and granuloma formation. Although viremia occurs, histologic lesions are not found uniformly throughout lymphoid tissues. We used experimental infection of cats with a highly pathogenic FIPV isolate, UCD8, to study histologic lesions, virus replication, and cytokine expression in multiple lymphoid tissues during the effusive phase of disease. Viral RNA was found in 76% of central tissues (mediastinal lymph node, spleen, mesenteric lymph node) examined, as compared to 27% of peripheral tissues (popliteal lymph node, cervical lymph node, femoral bone marrow). All tissues positive for virus replication also demonstrated lymphoid depletion. Generally, affected tissues had lower levels of IL-4 and IL-12–p40 mRNA and higher levels of IL-10 mRNA. Although no differences in IFN-γ or TNF-α mRNA were measured, TNF-α protein expression was greater in affected tissues and demonstrated a shift in the source of TNF-α from macrophages to lymphocytes. Together, these results colocalize FIPV replication, lymphocyte depletion in tissues, and alterations in cytokine transcription and translation. A possible role for TNF-α in the previously described FIPV-induced lymphocyte apoptosis is also suggested.
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