DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH).
DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH).
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DOI：
10.3390/ijms9101944
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发表时间：
2008-10
影响因子：
5.6
通讯作者：
Vieira, Maria J.
中科院分区：
文献类型：
--
作者：
Cerqueira, Laura;Azevedo, Nuno F.;Almeida, Carina;Jardim, Tatiana;Keevil, Charles William;Vieira, Maria J.
荧光原位杂交（FISH）是一种成熟的技术，用于各种目的，从临床诊断中的病原体检测到干细胞研究中的染色体稳定性测定。FISH的关键步骤涉及核酸区域的检测，因此，DNA分子通常用于探测感兴趣的序列。然而，自世纪之交以来，越来越多的实验室开始转向更稳健的DNA模拟方法，最值得注意的是肽和锁核酸（PNA和LNA）。在这篇综述中，我们将介绍不同的DNA模拟物作为单个微生物细胞存在的有效标记物的应用，并考虑它们的潜在优势和缺陷。然后使用rRNA数据库在灵敏度和特异性方面重新评估可用的PNA探针。此外，我们还试图预测DNA模拟物在试图原位检测特定核酸序列低拷贝数的已知技术中的适用性，例如催化报告基因沉积（CARD）和单个基因识别（RING）FISH。
Fluorescence in situ hybridization (FISH) is a well-established technique that is used for a variety of purposes, ranging from pathogen detection in clinical diagnostics to the determination of chromosomal stability in stem cell research. The key step of FISH involves the detection of a nucleic acid region and as such, DNA molecules have typically been used to probe for the sequences of interest. However, since the turn of the century, an increasing number of laboratories have started to move on to the more robust DNA mimics methods, most notably peptide and locked nucleic acids (PNA and LNA). In this review, we will cover the state-of-the-art of the different DNA mimics in regard to their application as efficient markers for the presence of individual microbial cells, and consider their potential advantages and pitfalls. Available PNA probes are then reassessed in terms of sensitivity and specificity using rRNA databases. In addition, we also attempt to predict the applicability of DNA mimics in well-known techniques attempting to detect in situ low number of copies of specific nucleic acid sequences such as catalyzed reporter deposition (CARD) and recognition of individual genes (RING) FISH.
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影响因子：
2.7
作者：
Braganca, S. M.;Azevedo, N. F.;Vieira, M. J.
通讯作者：
Vieira, M. J.
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