Protective Mechanism of Polygonum perfoliatum L. Extract on Chronic Alcoholic Liver Injury Based on UHPLC-QExactive Plus Mass Spectrometry Lipidomics and MALDI-TOF/TOF Mass Spectrometry Imaging.
Protective Mechanism of Polygonum perfoliatum L. Extract on Chronic Alcoholic Liver Injury Based on UHPLC-QExactive Plus Mass Spectrometry Lipidomics and MALDI-TOF/TOF Mass Spectrometry Imaging.
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基于UHPLC-QExactive Plus质谱脂质组学和MALDI-TOF/TOF质谱成像的穿叶蓼提取物对慢性酒精性肝损伤的保护机制
DOI：
10.3390/foods11111583
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发表时间：
2022-05-28
期刊：
影响因子：
5.2
通讯作者：
Zhou, Xin
中科院分区：
文献类型：
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作者：
Chen, Huaguo;Peng, Lei;Zhao, Chao;Cai, Zongwei;Zhou, Xin
关键词：
perfoliatum L.具有悠久的药用和食用应用历史。研究表明，它可以显著保护肝损伤，但其机制尚不清楚。本研究旨在从脂质代谢的角度探讨perfolatum对慢性酒精性肝损伤的保护机制。酒精暴露8周后，雄性Wister小鼠血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）水平显著升高，肝脏中乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）活性显著降低。同时，通过组织病理学观察小鼠肝组织的病理变化。然后，建立了超高效液相色谱（UHPLC） QExactive Plus质谱脂质组学和基质辅助激光解吸/电离飞行时间/飞行时间（MALDI-TOF/TOF）质谱成像方法来分析小鼠脂质代谢。通过统计分析鉴定出10种不同的脂质，包括脂肪酰基、甘油磷脂、丙烯醇脂质和鞘磷脂。经不同剂量（25 ~ 100 mg/kg）的perfolatium提取物干预后，血清中AST、ALT、ALP水平以及肝脏中ADH和ALDH活性均得到显著改善。肝索结构清晰，肝细胞排列紧密，未见其他明显异常。非目标脂质组学分析表明，perfolium提取物可以调节连续酒精暴露引起的10种不同脂质的代谢紊乱。途径分析提示，perfolatum提取物对慢性酒精性肝损伤的作用机制可能与调节亚油酸和α-亚麻酸有关。
Polygonum perfoliatum L. has a long history of medicinal and edible applications. Studies have shown that it can significantly protect liver injury, but the mechanism is unclear. The purpose of this study was to explore the protective mechanism of P. perfoliatum on chronic alcoholic liver injury from the perspective of lipid metabolism. After 8 weeks of alcohol exposure in male Wister mice, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in serum were significantly increased, and the activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in liver were significantly decreased. Meanwhile, pathological changes of liver tissue in mice were observed by histopathology. Then, Ultra-High Performance Liquid Chromatography (UHPLC) QExactive Plus Mass Spectrometer lipidomics and matrix-assisted laser desorption/ionization time-of-flight/time -of-flight (MALDI-TOF/TOF) mass spectrometry imaging methods were established to analyze lipid metabolism in mice. Ten different lipids were identified by statistical analysis, including Fatty Acyls, Glycerophospholipids, Prenol lipids and Sphingomyelins. After intervention with P. perfoliatum extracts at different doses (25 to 100 mg/kg), levels of AST, ALT, ALP in serum, and activities of ADH and ALDH in liver were significantly corrected. The hepatic cord structure was clear, and the liver cells were closely arranged without other obvious abnormalities. Non-target lipidomics analysis showed that P. perfoliatum extract could regulate the metabolic disorders of the 10 different lipids caused by continuous alcohol exposure. Pathway analysis suggested that the mechanism of P. perfoliatum extract on chronic alcoholic liver injury may be related to the regulation of linoleic acid and α-linolenic acid.
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影响因子：
4.2
作者：
Frazier, Thomas H;Stocker, Abigail M;McClain, Craig J
通讯作者：
McClain, Craig J
DOI：
10.31146/1682-8658-ecg-174-2-4-28
发表时间：
2020-01-01
期刊：
Experimental and Clinical Gastroenterology Journal
影响因子：
--
作者：
Lazebnik, L.B.;Golovanova, E.V.;Tsyganova, Yu. V.
通讯作者：
Tsyganova, Yu. V.
影响因子：
0.7
作者：
Fan D;Zhao Y;Zhou X;Gong X;Zhao C
通讯作者：
Zhao C
DOI：
10.1007/978-1-0716-1460-0_24
发表时间：
2021-01-01
期刊：
MYCOBACTERIA PROTOCOLS, 4 EDITION
影响因子：
--
作者：
Layre, Emilie
通讯作者：
Layre, Emilie
影响因子：
14.8
作者：
通讯作者：
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