A technology of a different sort: microraft arrays.
A technology of a different sort: microraft arrays.
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DOI：
10.1039/d1lc00506e
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发表时间：
2021-09-07
期刊：
影响因子：
6.1
通讯作者：
Allbritton NL
中科院分区：
文献类型：
--
作者：
Cortés-Llanos B;Wang Y;Sims CE;Allbritton NL
在整个生物医学研究中执行的一个常见程序是从混合种群中选择和分离生物实体，如细胞器、细胞和类有机物。在这篇综述中，我们描述了微阵列的发展和应用，这是一个分析和分离平台，可以在分离生物实体时使用广泛的标准和策略。微孔阵列由弹性微孔组成，弹性微孔带有可拆卸的聚合物基座(微孔)，在细胞分离过程中充当捕获和培养场所以及支持载体。该技术简单易行，可用于拥有数千万对象的样本，为单细胞RNA测序、亚细胞细胞器捕获和分析、高通量筛选和开发CRISPR基因编辑的细胞系以及有机物操作和选择等应用提供了一个灵活的平台。透明阵列与多种成像形态兼容，使得能够基于2D或3D空间表型或时间属性进行选择。每个微管可以根据需要单独隔离，并保持高活性，因为在微管的释放、捕获和沉积过程中，对细胞施加了接近于零的流体动应力。该平台已被用作标准显微镜的简单手动附件，或被整合到实现最先进成像算法和机器学习的全自动仪器中。大量的选择标准使得传统分选方法不可能实现分离，因此在生物和制药科学中引起了广泛的兴趣。
A common procedure performed throughout biomedical research is the selection and isolation of biological entities such as organelles, cells and organoids from a mixed population. In this review, we describe the development and application of microraft arrays, an analysis and isolation platform which enables a vast range of criteria and strategies to be used when separating biological entities. The microraft arrays are comprised of elastomeric microwells with detachable polymer bases (microrafts) that act as capture and culture sites as well as supporting carriers during cell isolation. The technology is elegant in its simplicity and can be implemented for samples possessing tens to millions of objects yielding a flexible platform for applications such as single-cell RNA sequencing, subcellular organelle capture and assay, high-throughput screening and development of CRISPR gene-edited cell lines, and organoid manipulation and selection. The transparent arrays are compatible with a multitude of imaging modalities enabling selection based on 2D or 3D spatial phenotypes or temporal properties. Each microraft can be individually isolated on demand with retention of high viability due to the near zero hydrodynamic stress imposed upon the cells during microraft release, capture and deposition. The platform has been utilized as a simple manual add-on to a standard microscope or incorporated into fully automated instruments that implement state-of-the-art imaging algorithms and machine learning. The vast array of selection criteria enables separations not possible with conventional sorting methods, thus garnering widespread interest in the biological and pharmaceutical sciences.
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影响因子：
14
作者：
Gach, Philip C.;Sims, Christopher E.;Allbritton, Nancy L.
通讯作者：
Allbritton, Nancy L.
影响因子：
48
作者：
Dong X;Zhang L;Milholland B;Lee M;Maslov AY;Wang T;Vijg J
通讯作者：
Vijg J
DOI：
10.1139/o96-080
发表时间：
1996-01-01
期刊：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
影响因子：
--
作者：
Chiquet, M;Matthisson, M;ChiquetEhrismann, R
通讯作者：
ChiquetEhrismann, R
影响因子：
7.4
作者：
Gach, Philip C.;Attayek, Peter J.;Herrera, Gabriela;Yeh, Jen Jen;Allbritton, Nancy L.
通讯作者：
Allbritton, Nancy L.
影响因子：
7.4
作者：
Gach, Philip C.;Xu, Wei;Allbritton, Nancy L.
通讯作者：
Allbritton, Nancy L.