Mucus glycoconjugate complexes released from feline trachea by a bacterial toxin.
Mucus glycoconjugate complexes released from feline trachea by a bacterial toxin.
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细菌毒素从猫科动物气管释放的粘液糖复合物。
DOI：
10.1165/ajrcmb.12.3.7873196
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发表时间：
1995
影响因子：
6.4
通讯作者：
J. Sheehan
中科院分区：
文献类型：
--
作者：
D. C. Fung;M. Somerville;P. Richardson;J. Sheehan
本文描述了由铜绿假单胞菌毒素 dirhamnolipid (DRL) 从猫气管释放的低密度粘液糖复合物。猫气管中的粘液糖缀合物在体内用 3H-脯氨酸和 14C-葡萄糖进行放射性标记。将对照粘液和200微克/ml DRL释放的粘液溶解在盐酸胍缓冲液(GuHCl)中并在Sepharose CL-2B上进行色谱分离。通过在 CsCl/GuHCl 中等密度梯度离心分离在柱的空隙体积 (V0) 中洗脱的分子。所有样品均在 rho = 约 1.50 和约 1.60 g/ml 时给出放射性标记和高碘酸/希夫 (PAS) 反应物质的峰，但 DRL 刺激的样品含有低密度物质 (rho < 1.32 g/ml)，也是 PAS 反应和放射性标记的。体外与 DRL 一起温育的对照分泌物未形成低密度材料。在 Triton X-100（1% vol/vol）（一种非离子洗涤剂）中，低密度材料表现为较小的分子，在 Sepharose CL-2B 柱的部分包含体积 (Vi) 中运行，但仍在 Sepharose S-300 的 V0 中运行。与软骨素酶 ABC、肝素酶 II 和 III 以及角蛋白酶一起孵育未能改变其在 S-300 上的洗脱曲线，这是反对糖胺聚糖的证据；但用胰蛋白酶或蛋白酶 K 进行蛋白水解会产生两个峰，即接近柱总体积的肽片段和 V0 中的糖肽。 V0 糖肽在 CsCl 梯度中条带在 1.50 至 1.55 g/ml 之间，并在 Sephacryl S-400 的 Vi 中作为单峰洗脱，表明是明显均质的糖肽，小于正常粘蛋白的糖肽。该糖肽中主要的 14C 标记糖是岩藻糖、葡萄糖胺、半乳糖胺和半乳糖，与粘蛋白一致。因此，DRL 释放稳定但非共价的复合物，其中含有一种或多种不同的粘蛋白样糖缀合物，可能与脂质和肽结合。我们讨论它们与气道疾病（包括囊性纤维化）的可能相关性。
This paper describes low-density mucus glycoconjugates released from feline trachea by dirhamnolipid (DRL), a toxin from Pseudomonas aeruginosa. Mucus glycoconjugates in feline tracheas were radiolabeled in vivo with 3H-proline and 14C-glucose. Control mucus and that released by 200 micrograms/ml DRL were dissolved in guanidine hydrochloride buffer (GuHCl) and chromatographed on Sepharose CL-2B. Molecules eluting in the void volume (V0) of the column were isolated by isopycnic density gradient centrifugation in CsCl/GuHCl. All samples gave peaks of radiolabeled and periodic acid/Schiff (PAS)-reactive material at rho = approximately 1.50 and approximately 1.60 g/ml, but DRL-stimulated samples contained low-density material (rho < 1.32 g/ml), also PAS-reactive and radiolabeled. Control secretions incubated with DRL in vitro did not form low-density material. In Triton X-100 (1% vol/vol), a nonionic detergent, low-density material behaved as smaller molecules, running in the partially included volume (Vi) of the column of Sepharose CL-2B, but still in the V0 of Sephacryl S-300. Incubation with chondroitinase ABC, heparinase II and III, and keratanase failed to change its elution profile on S-300, evidence against glycosaminoglycans; but proteolysis with trypsin or proteinase K gave two peaks, peptide fragments near the totally included volume of the column and glycopeptides in V0. The V0 glycopeptides banded between 1.50 and 1.55 g/ml in a CsCl gradient and eluted as a single peak in the Vi of Sephacryl S-400, suggesting a distinct homogeneous glycopeptide, smaller than those from normal mucins. The main 14C-labeled sugars in this glycopeptide were fucose, glucosamine, galactosamine, and galactose, consistent with a mucin. Thus, DRL releases stable but noncovalent complexes containing one or more distinct mucinlike glycoconjugates, probably combined with lipids and peptides. We discuss their possible relevance to airway diseases, including cystic fibrosis.
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DOI：
--
发表时间：
1993-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Bobek;Hsiaoyun Tsai;A. R. Biesbrock;Michael J. Levine
通讯作者：
L. Bobek;Hsiaoyun Tsai;A. R. Biesbrock;Michael J. Levine
人类和犬气道粘膜体外产生的分泌物的密度梯度分析：此类分泌物中脂质和蛋白聚糖的鉴定。
影响因子：
1.7
作者：
Bhaskar,KR;O'Sullivan,DD;Opaskar-Hincman,H;Reid,LM;Coles,SJ
通讯作者：
Coles,SJ
培养物中牛气管浆液细胞分泌的糖缀合物。
影响因子：
3.9
作者：
Paul,A;Picard,J;Mergey,M;Veissiere,D;Finkbeiner,WE;Basbaum,CB
通讯作者：
Basbaum,CB
支气管上皮粘液的大分子和脂质成分。
DOI：
--
发表时间：
1989
期刊：
Symposia of the Society for Experimental Biology
影响因子：
--
作者：
Reid,LM;Bhaskar,KR
通讯作者：
Bhaskar,KR
培养的狗气管上皮细胞合成硫酸化大分子糖复合物，并在暴露于细胞外蛋白酶时从细胞表面释放它们。
影响因子：
1.7
作者：
Varsano,S;Basbaum,CB;Forsberg,LS;Borson,DB;Caughey,G;Nadel,JA
通讯作者：
Nadel,JA