Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects.
Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects.
复制标题
DOI：
10.1038/s41598-017-13586-8
复制
发表时间：
2017-10-19
影响因子：
4.6
通讯作者：
Bai Y
中科院分区：
文献类型：
--
作者：
Li Z;Pan J;Ma J;Zhang Z;Bai Y
对于因保留骨膜的肿瘤而接受部分或全部下颌骨切除术的患者，可能会发生自发骨再生，以重建下颌骨的连续性。然而，关于骨膜在骨再生中的精确作用的数据很少。因此，我们的目的是研究参与下颌骨再生的骨膜基因表达。在六只小型猪中制造下颌节段缺损，并保留骨膜。在第1周和第2周时收获缺损部位和对照部位的骨膜。基因本体（GO）分析显示，与未成熟伤口愈合相关的机制在第 1 周明显上调。相比之下，到第 2 周，骨骼发育、骨化和骨矿化的 GO 类别在第 2 周显着过度表达，其中有几个编码细胞分化、细胞外基质形成和解剖结构发育的基因。此外，本研究中Tgfβ/Bmp、Wnt和Notch信号均与成骨过程相关。除了成骨作用外，与血管生成和神经发生相关的基因在第 2 周也很突出。这些发现揭示了参与骨再生的骨膜细胞的基因表达谱在不同时间点有所不同，并提出了膜内骨再生早期愈合阶段差异表达的候选基因的数量。
Spontaneous bone regeneration could occur to reestablish mandibular bony continuity in patients who underwent partial or total mandibulectomy for tumors with periosteum-preserving. However, scarce data is available related to the precise role of periosteum in this bone regeneration. Therefore we aimed to investigate the gene expression of periosteum that were involved in the mandibular bone regeneration. Mandibular segmental defects were created in six mini-pigs with periosteum preserved. The periosteum of defects and control site were harvested at 1 and 2 weeks. Gene ontology (GO) analysis showed that the mechanisms concerning immature wound healing were clearly up-regulated at week 1. In contrast, by week-2, the GO categories of skeletal development, ossification and bone mineralization were significantly over-represented at week-2 with several genes encoding cell differentiation, extracellular matrix formation, and anatomical structure development. Furthermore, Tgfβ/Bmp, Wnt and Notch signaling were all related to the osteogenic process in this study. Besides osteogenesis, genes related to angiogenesis and neurogenesis were also prominent at week-2. These findings revealed that the gene expression profile of the periosteum’s cells participating in bone regeneration varied in different time points, and numbers of candidate genes that differentially expressed during early healing stages of intramembranous bone regeneration were suggested.
登录
查看更多内容
影响因子：
15.8
作者：
Chen Y;Whetstone HC;Lin AC;Nadesan P;Wei Q;Poon R;Alman BA
通讯作者：
Alman BA
影响因子：
1.2
作者：
Ito, Ryohei;Matsumiya, Tomoh;Kimura, Hiroto
通讯作者：
Kimura, Hiroto
影响因子：
4.3
作者：
Ivanovski, S.;Hamlet, S.;Donos, N.
通讯作者：
Donos, N.
影响因子：
3.7
作者：
Bais M;McLean J;Sebastiani P;Young M;Wigner N;Smith T;Kotton DN;Einhorn TA;Gerstenfeld LC
通讯作者：
Gerstenfeld LC
影响因子：
6.4
作者：
Kolar, Paula;Schmidt-Bleek, Katharina;Duda, Georg N.
通讯作者：
Duda, Georg N.