Contrasting influences of Drosophila white/mini-white on ethanol sensitivity in two different behavioral assays.
Contrasting influences of Drosophila white/mini-white on ethanol sensitivity in two different behavioral assays.
复制标题
DOI：
10.1111/acer.12421
复制
发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Grotewiel M
中科院分区：
文献类型：
--
作者：
Chan RF;Lewellyn L;DeLoyht JM;Sennett K;Coffman S;Hewitt M;Bettinger JC;Warrick JM;Grotewiel M
果蝇黑腹果蝇被广泛用于研究酒精相关行为的遗传机制。过去的许多果蝇研究，包括我们实验室的研究，都使用携带遗传表型标记mini-white的转座子来操纵基因表达，mini-white是内源性基因white的衍生物。迷你白色转基因标记或内源性白色基因是否影响果蝇对急性乙醇暴露的行为反应尚未有系统的研究。我们通过(i)带有mini-white标记的转座子，（ii）针对mini-white和white的RNAi和（iii） white的空等位基因来操纵mini-white和white的表达。我们使用先前描述的eRING试验（基于乙醇存在下的攀爬）和基于乙醇诱导镇静的试验来评估乙醇敏感性和耐受性。在eRING实验中，乙醇诱导的攀爬损伤与一系列转座子插入的迷你白色标记的表达呈负相关。此外，在eRING试验中，携带白色零等位基因的果蝇或携带rnai介导的mini-white基因敲低的果蝇对乙醇的敏感性明显高于表达内源性白色或mini-white标记的对照组。相比之下，乙醇镇静实验中测量的乙醇敏感性和快速耐受性不受果蝇微白或内源性白表达减少的影响。在erin实验中测量的乙醇敏感性明显受到白色和迷你白色的影响，这使得erin在使用带有迷你白色标记的转基因研究果蝇酒精相关行为时存在问题。相比之下，本文描述的乙醇镇静实验是研究果蝇乙醇镇静和快速耐受性的合适行为范例，包括那些使用广泛使用的标记为mini-white的转基因的果蝇。
The fruit fly Drosophila melanogaster has been used extensively to investigate genetic mechanisms of ethanol-related behaviors. Many past studies in flies, including studies from our laboratory, have manipulated gene expression using transposons carrying the genetic-phenotypic marker mini-white, a derivative of the endogenous gene white. Whether the mini-white transgenic marker or the endogenous white gene influence behavioral responses to acute ethanol exposure in flies has not been systematically investigated. We manipulated mini-white and white expression via (i) transposons marked with mini-white, (ii) RNAi against mini-white and white and (iii) a null allele of white. We assessed ethanol sensitivity and tolerance using a previously described eRING assay (based on climbing in the presence of ethanol) and an assay based on ethanol-induced sedation. In eRING assays, ethanol-induced impairment of climbing correlated inversely with expression of the mini-white marker from a series of transposon insertions. Additionally, flies harboring a null allele of white or flies with RNAi-mediated knockdown of mini-white were significantly more sensitive to ethanol in eRING assays than controls expressing endogenous white or the mini-white marker. In contrast, ethanol sensitivity and rapid tolerance measured in the ethanol sedation assay were not affected by decreased expression of mini-white or endogenous white in flies. Ethanol sensitivity measured in the eRING assay is noticeably influenced by white and mini-white, making eRING problematic for studies on ethanol-related behavior in Drosophila using transgenes marked with mini-white. In contrast, the ethanol sedation assay described here is a suitable behavioral paradigm for studies on ethanol sedation and rapid tolerance in Drosophila including those that use widely available transgenes marked with mini-white.
登录
查看更多内容
影响因子：
1.9
作者：
Anaka, Matthew;MacDonald, C. Danielle;Lloyd, Vett
通讯作者：
Lloyd, Vett
影响因子：
2
作者：
Diegelmann, S;Zars, M;Zars, T
通讯作者：
Zars, T
DOI：
10.1097/01.alc.0000141817.15993.98
发表时间：
2004-10-01
影响因子：
3.2
作者：
Berger, KH;Heberlein, U;Moore, MS
通讯作者：
Moore, MS
影响因子：
3.7
作者：
Lasek AW;Lim J;Kliethermes CL;Berger KH;Joslyn G;Brush G;Xue L;Robertson M;Moore MS;Vranizan K;Morris SW;Schuckit MA;White RL;Heberlein U
通讯作者：
Heberlein U
DOI：
10.1523/jneurosci.1944-12.2012
发表时间：
2012-12-05
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Peru Y Colón de Portugal RL;Acevedo SF;Rodan AR;Chang LY;Eaton BA;Rothenfluh A
通讯作者：
Rothenfluh A