Genome-wide association study reveals a patatin-like lipase relating to the reduction of seed oil content in Brassica napus.
Genome-wide association study reveals a patatin-like lipase relating to the reduction of seed oil content in Brassica napus.
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DOI：
10.1186/s12870-020-02774-w
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发表时间：
2021-01-06
影响因子：
5.3
通讯作者：
Jiang L
中科院分区：
文献类型：
--
作者：
Wang H;Wang Q;Pak H;Yan T;Chen M;Chen X;Wu D;Jiang L
甘蓝型油菜（Brassica napus L.）油菜是世界上广泛种植的重要油料作物，种子含油量是油菜最重要的性状之一。为了增加SOC，先前已经进行了许多努力来促进脂质生物合成途径上的基因的功能。然而，种子油的形成是脂质合成和分解之间的动态平衡。因此，削弱或消除参与脂质降解的基因的功能以获得更高的最终SOC也是合理的。我们应用全基因组关联研究（GWAS）对290份核心种质资源的SOC进行了研究。在GWAS中总共使用了2，705，480个高质量的SNP，我们确定了BnaC 07 g30920 D，一个与SOC相关的patatin样脂肪酶（PTL）基因。特别是，BnaC 07 g30920 D启动子区域的6个单核苷酸多态性（SNP）与SOC的显著降低相关，导致SOC降低4.7-6.2%。我们进行了计算机模拟分析，共显示了40个PTL，分为4个分支，均匀分布在甘蓝型油菜的A和C亚基因组上。RNA-seq分析表明，BnPTLs在生殖组织中优先表达，尤其是在成熟种子中。我们使用GWAS鉴定了与SOC相关的BnPTL基因BnaC 07 g30920 D，并对甘蓝型油菜中的40个PTL进行了计算机分析。本研究结果丰富了油菜SOC形成的相关知识，为进一步研究BnPTL基因的功能奠定了基础。在线版本包含补充材料，可通过10.1186/s12870-020-02774-w获得。
Rapeseed (Brassica napus L.) is an important oil crop world-widely cultivated, and seed oil content (SOC) is one of the most important traits for rapeseed. To increase SOC, many efforts for promoting the function of genes on lipid biosynthesis pathway have been previously made. However, seed oil formation is a dynamic balance between lipid synthesis and breakdown. It is, therefore, also reasonable to weaken or eliminate the function of genes involved in lipid degradation for a higher final SOC. We applied a genome-wide association study (GWAS) on SOC in a collection of 290 core germplasm accessions. A total of 2,705,480 high-quality SNPs were used in the GWAS, and we identified BnaC07g30920D, a patatin-like lipase (PTL) gene, that was associated with SOC. In particular, six single-nucleotide-polymorphisms (SNPs) in the promoter region of BnaC07g30920D were associated with the significant reduction of SOC, leading to a 4.7–6.2% reduction of SOCs. We performed in silico analysis to show a total of 40 PTLs, which were divided into four clades, evenly distributed on the A and C subgenomes of Brassica napus. RNA-seq analysis unveiled that BnPTLs were preferentially expressed in reproductive tissues especially maturing seeds. We identified BnaC07g30920D, a BnPTL gene, that was associated with SOC using GWAS and performed in silico analysis of 40 PTLs in Brassica napus. The results enrich our knowledge about the SOC formation in rapeseed and facilitate the future study in functional characterization of BnPTL genes. The online version contains supplementary material available at 10.1186/s12870-020-02774-w.
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影响因子：
6.9
作者：
Chia, TYP;Pike, MJ;Rawsthorne, S
通讯作者：
Rawsthorne, S
影响因子：
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作者：
GALLIARD, T
通讯作者：
GALLIARD, T
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作者：
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作者：
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通讯作者：
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