Patatin-related phospholipase pPLAIIIδ influences auxin-responsive cell morphology and organ size in Arabidopsis and Brassica napus.
Patatin-related phospholipase pPLAIIIδ influences auxin-responsive cell morphology and organ size in Arabidopsis and Brassica napus.
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Patatin 相关磷脂酶 pPLAIII delta 影响拟南芥和甘蓝型油菜中生长素响应细胞形态和器官大小
DOI：
10.1186/s12870-014-0332-1
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发表时间：
2014-11-27
影响因子：
5.3
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Dong Y;Li M;Zhang P;Wang X;Fan C;Zhou Y
patatin 相关磷脂酶亚家族 III (pPLAIIIs) 的成员与生长素反应有关。然而，尚不清楚这些基因是否以及如何影响植物和细胞形态发生。在这里，我们研究了 patatin 相关磷脂酶 pPLAIIIδ 在拟南芥和甘蓝型油菜生长素响应细胞形态和器官大小中的作用。我们发现，在拟南芥和甘蓝型油菜的大多数器官中，pPLAIIIδ 的过度表达抑制纵向生长，但促进横向生长。与野生型植物相比，pPLAIIIδ-KO植物表现出下胚轴细胞伸长增强，pPLAIIIδ-OE植物表现出下胚轴径向细胞生长变宽和叶面细胞极性降低。对于pPLAIIIδ突变体中的下胚轴表型，类似于对乙烯的“三重反应”，我们检查了参与乙烯生物合成的ACS和ACO基因的表达，发现与WT植物相比，两个OE品系中的ACS4和ACS5平均上调2.5倍。 pPLAIIIδ 表达改变的植物中内源生长素的分布受到干扰。 pPLAIIIδ-OE 和 KO 植物在光照和黑暗条件下对吲哚-3-乙酸促进的下胚轴伸长表现出不同的敏感性。在黑暗中对生长素诱导基因的基因表达分析表明，用 1 μM IAA 处理 12 小时后，与 WT 和 KO 植物相比，OE 植物保持了更高的生长素响应。在光照下用 10 μM IAA 处理 30 分钟后，两个 OE 植物系中早期生长素诱导的基因显着上调。这些数据表明 PLAIIIδ 基因通过参与植物生长素分布的调节而在细胞形态和器官大小中发挥重要作用。本文的在线版本 (doi:10.1186/s12870-014-0332-1) 包含补充材料，可供授权用户使用。
The members of the patatin-related phospholipase subfamily III (pPLAIIIs) have been implicated in the auxin response. However, it is not clear whether and how these genes affect plant and cell morphogenesis. Here, we studied the roles of the patatin-related phospholipase pPLAIIIδ in auxin-responsive cell morphology and organ size in Arabidopsis and Brassica napus. We show that overexpression of pPLAIIIδ inhibited longitudinal growth but promoted transverse growth in most organs of Arabidopsis and Brassica napus. Compared to wild-type plants, pPLAIIIδ-KO plants exhibited enhanced cell elongation in hypocotyls, and pPLAIIIδ-OE plants displayed broadened radial cell growth of hypocotyl and reduced leaf pavement cell polarity. For the hypocotyl phenotype in pPLAIIIδ mutants, which resembles the “triple response” to ethylene, we examined the expression of the ACS and ACO genes involved in ethylene biosynthesis and found that ACS4 and ACS5 were up-regulated by 2.5-fold on average in two OE lines compared with WT plants. The endogenous auxin distribution was disturbed in plants with altered pPLAIIIδ expression. pPLAIIIδ-OE and KO plants exhibited different sensitivities to indole-3-acetic acid-promoted hypocotyl elongation in both light and dark conditions. Gene expression analysis of auxin-induced genes in the dark showed that OE plants maintained a higher auxin response compared with WT and KO plants after treatment with 1 μM IAA for 12 h. Following treatment with 10 μM IAA for 30 min in the light, early auxin-induced genes were significantly up-regulated in two OE plant lines. These data suggest that the PLAIIIδ gene plays an important role in cell morphology and organ size through its involvement in the regulation of auxin distribution in plants. The online version of this article (doi:10.1186/s12870-014-0332-1) contains supplementary material, which is available to authorized users.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
64.5
作者：
Geldner, N;Anders, N;Jürgens, G
通讯作者：
Jürgens, G
DOI：
10.1016/j.cub.2009.08.052
发表时间：
2009-11-17
期刊：
Current biology : CB
影响因子：
--
作者：
Fu Y;Xu T;Zhu L;Wen M;Yang Z
通讯作者：
Yang Z
影响因子：
56.9
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
通讯作者：
Kay, SA
影响因子：
64.8
作者：
Jaillais, Yvon;Fobis-Loisy, Isabelle;Gaude, Thierry
通讯作者：
Gaude, Thierry