Dynamic photoelectrical regulation of ECM protein and cellular behaviors.
Dynamic photoelectrical regulation of ECM protein and cellular behaviors.
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DOI：
10.1016/j.bioactmat.2022.09.022
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发表时间：
2023-04
影响因子：
18.9
通讯作者：
Cheng, Kui
中科院分区：
文献类型：
--
作者：
Wang, Xiaozhao;Yao, Cai;Yao, Xudong;Lin, Junxin;Li, Rui;Huang, Kun;Lin, Weiming;Long, Xiaojun;Dai, Chao;Dong, Jiajun;Yu, Xuegong;Huang, Wenwen;Weng, Wenjian;Wang, Qi;Ouyang, Hongwei;Cheng, Kui
细胞-细胞外基质（ECM）-材料相互作用的动态调节对于各种生物医学应用至关重要。本研究在单层石墨烯（Gr）/n-型硅衬底（Gr/Si）上建立了一种用于调控细胞附着/脱离行为的光激活分子开关。通过Gr/Si界面处的光照射引发，预吸附的蛋白质（牛血清白蛋白、ECM蛋白质胶原蛋白-1和纤连蛋白）经历质子化以通过π-π相互作用实现负电荷转移到Gr膜（n-掺杂）。这种n-掺杂过程刺激ECM蛋白的构象转换。这些ECM相互作用物的结构改变显著降低了细胞表面受体-配体相互作用的特异性（例如，整合素识别），导致细胞粘附的动态调节和最终的细胞脱离。RNA测序结果显示，从Gr/Si系统分离的骨髓间充质基质细胞片表现出调节免疫调节特性和增强成骨分化，这意味着它们在骨组织再生中的潜在应用。这项工作不仅提供了一种快速可行的方法，可控的细胞/细胞片收获，但也提供了新的见解细胞ECM材料通信的理解。基于Gr/Si系统的可见光控制的细胞脱离是通过直接切换整合素配体对纤连蛋白的识别来实现的。光照引发ECM蛋白的电子通过π-π相互作用转移到Gr层，这一电荷转移过程导致ECM蛋白构象发生变化，从而削弱了整合素与RGD的结合，最终导致细胞脱附。在（Gr/Si）基底上构建了一个调控细胞贴附/脱离行为的光激活分子开关。光诱导ECM蛋白通过π-π相互作用将电荷转移到Gr/Si上，导致ECM蛋白的构象改变。ECM的结构变化减弱了RGD和整合素之间的结合，诱导细胞脱离。这项工作提供了一种可行的方法，细胞收获和提高细胞ECM材料通信的理解。
Dynamic regulation of cell-extracellular matrix (ECM)-material interactions is crucial for various biomedical applications. In this study, a light-activated molecular switch for the modulation of cell attachment/detachment behaviors was established on monolayer graphene (Gr)/n-type Silicon substrates (Gr/Si). Initiated by light illumination at the Gr/Si interface, pre-adsorbed proteins (bovine serum albumin, ECM proteins collagen-1, and fibronectin) underwent protonation to achieve negative charge transfer to Gr films (n-doping) through π-π interactions. This n-doping process stimulated the conformational switches of ECM proteins. The structural alterations in these ECM interactors significantly reduced the specificity of the cell surface receptor-ligand interaction (e.g., integrin recognition), leading to dynamic regulation of cell adhesion and eventual cell detachment. RNA-sequencing results revealed that the detached bone marrow mesenchymal stromal cell sheets from the Gr/Si system manifested regulated immunoregulatory properties and enhanced osteogenic differentiation, implying their potential application in bone tissue regeneration. This work not only provides a fast and feasible method for controllable cells/cell sheets harvesting but also gives new insights into the understanding of cell-ECM-material communications. A visible light controlled cell detachment based on Gr/Si system is achieved by directly switching the recognition of fibronectin by integrin ligand. Initiated by light illumination, electrons from ECM proteins transferred to Gr layer through π-π interactions, this charge transfer process induced conformational changes of the ECM protein, which resultantly weakened the binding between RGD and integrin, and eventually induce cell detachment. A light-activated molecular switch for regulation of cell attachment/detachment behaviors was established on (Gr/Si) substrates. Light-induced charge transfer from ECM protein to Gr/Si through π-π interactions, resulting in the conformational alteration of ECM proteins. Structural changes in ECM weakened the binding between RGD and integrin, inducing cell detachment. This work provides a feasible method for cell harvesting and improves the understanding of cell-ECM-material communications.
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影响因子：
38.3
作者：
Das, A.;Pisana, S.;Sood, A. K.
通讯作者：
Sood, A. K.
通过 TiO2 纳米点薄膜的光响应调节蛋白质行为
影响因子：
4.6
作者：
Cheng K;Hong Y;Yu M;Lin J;Weng W;Wang H
通讯作者：
Wang H
具有不同二级结构特征的三种典型蛋白质在石墨烯上的吸附机理及诱导构象变化
影响因子：
3.9
作者：
Guo, Jingjing;Yao, Xiaojun;Liu, Huanxiang
通讯作者：
Liu, Huanxiang
影响因子：
9.5
作者：
Akdim, Brahim;Pachter, Ruth;Farmer, Barry L.
通讯作者：
Farmer, Barry L.
DOI：
10.1002/anie.201509025
发表时间：
2016-01-18
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Giner-Casares JJ;Henriksen-Lacey M;García I;Liz-Marzán LM
通讯作者：
Liz-Marzán LM