Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum.
Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum.
复制标题
极性蛋白 Wag31 既激活又抑制两极和隔膜的细胞壁代谢。
DOI：
10.3389/fmicb.2022.1085918
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发表时间：
2022
影响因子：
5.2
通讯作者：
Boutte, Cara C.
中科院分区：
文献类型：
--
作者：
Habibi Arejan, Neda;Ensinck, Delfina;Diacovich, Lautaro;Patel, Parthvi Bharatkumar;Quintanilla, Samantha Y.;Emami Saleh, Arash;Gramajo, Hugo;Boutte, Cara C.
关键词：
分枝杆菌细胞的伸长发生在细胞两极;然而，目前尚不清楚细胞壁插入是如何限制在两极或它是如何组织的。Wag 31是一种定位于两极的细胞质蛋白，对极性生长至关重要，但其分子功能尚未被描述。在这项研究中，我们使用丙氨酸扫描诱变，以确定参与细胞形态发生的Wag 31残基。我们的数据表明，Wag 31有助于控制适当的分隔以及新的和旧的极伸长。我们已经确定了参与这些基本功能的关键氨基酸残基。酶分析显示Wag 31通过调节酰基辅酶A羧化酶（ACCase）活性与脂质代谢相互作用。我们表明，Wag 31不控制极性生长，通过调节细胞壁前体酶的细胞内膜结构域的本地化，我们还表明，磷酸化的Wag 31不实质性调节肽聚糖代谢。这项工作建立了Wag 31在分枝杆菌细胞周期中的新的调节功能，并阐明了对Wag 31功能的新分子模型的需求。
Mycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this study we used alanine scanning mutagenesis to identify Wag31 residues involved in cell morphogenesis. Our data show that Wag31 helps to control proper septation as well as new and old pole elongation. We have identified key amino acid residues involved in these essential functions. Enzyme assays revealed that Wag31 interacts with lipid metabolism by modulating acyl-CoA carboxylase (ACCase) activity. We show that Wag31 does not control polar growth by regulating the localization of cell wall precursor enzymes to the Intracellular Membrane Domain, and we also demonstrate that phosphorylation of Wag31 does not substantively regulate peptidoglycan metabolism. This work establishes new regulatory functions of Wag31 in the mycobacterial cell cycle and clarifies the need for new molecular models of Wag31 function.
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影响因子：
7.7
作者：
Gupta KR;Gwin CM;Rahlwes KC;Biegas KJ;Wang C;Park JH;Liu J;Swarts BM;Morita YS;Rego EH
通讯作者：
Rego EH
影响因子：
7.7
作者：
García-Heredia A;Kado T;Sein CE;Puffal J;Osman SH;Judd J;Gray TA;Morita YS;Siegrist MS
通讯作者：
Siegrist MS
影响因子：
4.5
作者：
Eswaramoorthy, Prahathees;Winter, Peter W.;Ramamurthi, Kumaran S.
通讯作者：
Ramamurthi, Kumaran S.
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
3.9
作者：
HUNAITI, AR;KOLATTUKUDY, PE
通讯作者：
KOLATTUKUDY, PE