Integrated Transcriptomic and Proteomic Analysis Reveals Up-Regulation of Apoptosis and Small Heat Shock Proteins in Lens of Rats Under Low Temperature.
Integrated Transcriptomic and Proteomic Analysis Reveals Up-Regulation of Apoptosis and Small Heat Shock Proteins in Lens of Rats Under Low Temperature.
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DOI：
10.3389/fphys.2021.683056
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发表时间：
2021
影响因子：
4
通讯作者：
Shentu X
中科院分区：
文献类型：
--
作者：
Zhou J;Wu J;Zheng S;Chen X;Zhou D;Shentu X
冷性白内障是当温度降低时透镜可逆性混浊。然而，我们观察到，当大鼠透镜的温度在较低温度下保持较长时间时，透镜的混浊仅部分可逆。为探讨低温刺激下晶状体混浊不可逆部分的分子机制，我们采用比较转录组学和蛋白质组学方法，系统研究了低温刺激下大鼠透镜囊膜的分子变化。基于RNA测序的转录组学分析显示低温组中与透镜结构和发育相关的基因显著上调。Hub基因是小分子热休克蛋白（sHSPs）。除了与转录组学结果相同的发现外，基于液相色谱-串联质谱的蛋白质组学分析也揭示了凋亡过程的上调。为了进一步分析这一过程中的调控机制，我们随后进行了综合分析，确定了Notch 3/Hes 1和PI 3 K/Akt/Xip信号轴的下调。本研究通过转录组学和蛋白质组学数据揭示了冷刺激下大鼠透镜中凋亡过程的激活，以及sHSP相关的热休克反应作为一种潜在的保护因子。
Cold cataract is the reversible opacification of the lens when the temperature decreases. However, we observed that when temperature of the rats’ lens was maintained at a lower temperature for a prolonged time, the opacification of lens was only partly reversible. To review the potential molecular mechanism of the irreversible part of opacification under cold stimulation, we applied comparative transcriptomic and proteomic analysis to systematically investigate the molecular changes that occurred in the lens capsules of rats under low temperature treatments. The RNA sequencing based transcriptomic analysis showed a significant up-regulation of genes related to the lens structure and development in the Hypothermia Group. Hub genes were small heat shock proteins (sHSPs). Besides the same findings as the transcriptomic results, the liquid chromatography-tandem mass spectrometry based proteomic analysis also revealed the up-regulation of the apoptotic process. To further analyze the regulatory mechanism in this process, we subsequently performed integrated analysis and identified the down-regulation of Notch3/Hes1 and PI3K/Akt/Xiap signaling axis. Our research revealed the activation of the apoptotic process in rats’ lens under cold stimulation, and the sHSP related heat shock response as a potential protective factor through our transcriptomic and proteomic data.
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影响因子：
3.4
作者：
Cui, Gangfeng;Wang, Ledan;Huang, Wenjuan
通讯作者：
Huang, Wenjuan
影响因子：
7.4
作者：
Li, WC;Spector, A
通讯作者：
Spector, A
影响因子：
2
作者：
Bermudez, Maria A.;Vicente, Ana F.;Gonzalez, Francisco
通讯作者：
Gonzalez, Francisco
影响因子：
3.4
作者：
Kumari, Sindhu S.;Gandhi, Jason;Mustehsan, Mohammed H.;Eren, Semih;Varadaraj, Kulandaiappan
通讯作者：
Varadaraj, Kulandaiappan
DOI：
10.1016/j.biocel.2012.06.012
发表时间：
2012-10-01
影响因子：
4
作者：
de Thonel, Aurelie;Le Mouel, Anne;Mezger, Valerie
通讯作者：
Mezger, Valerie