Cutting Edge: Distinct B Cell Repertoires Characterize Patients with Mild and Severe COVID-19.
Cutting Edge: Distinct B Cell Repertoires Characterize Patients with Mild and Severe COVID-19.
复制标题
DOI：
10.4049/jimmunol.2100135
复制
发表时间：
2021-06-15
期刊：
影响因子：
--
通讯作者：
Kleinstein SH
中科院分区：
文献类型：
--
作者：
Hoehn KB;Ramanathan P;Unterman A;Sumida TS;Asashima H;Hafler DA;Kaminski N;Dela Cruz CS;Sealfon SC;Bukreyev A;Kleinstein SH
针对 COVID-19 的保护性免疫力可能取决于感染或接种疫苗后 SARS-CoV-2 特异性浆细胞和记忆 B 细胞的产生。先前的研究发现，严重的 COVID-19 中生发中心反应受到干扰。这可能会对针对再次感染的长期免疫力产生不利影响。与滤泡外 B 细胞反应一致，重症 COVID-19 患者的克隆扩增、类别转换、未突变浆母细胞的频率升高。然而，尚不清楚轻度 COVID-19 个体的 B 细胞群是否也存在类似的偏差。在这里，我们使用 B 细胞的单细胞 RNA 测序来表明，与重症 COVID-19 患者相比，症状轻微的 COVID-19 受试者在症状出现约 30 天后，其 B 细胞库富含克隆多样性、体细胞高度突变的记忆 B 细胞。这提供了证据，表明 B 细胞反应在轻度 COVID-19 中较少受到干扰，并导致记忆 B 细胞的产生。
Protective immunity against COVID-19 likely depends on the production of SARS-CoV-2 specific plasma cells and memory B cells after infection or vaccination. Previous work has found that germinal center reactions are disrupted in severe COVID-19. This may adversely affect long-term immunity against re-infection. Consistent with an extrafollicular B cell response, patients with severe COVID-19 have elevated frequencies of clonally expanded, class switched, unmutated plasmablasts. However, it is unclear whether B cell populations in individuals with mild COVID-19 are similarly skewed. Here, we use single cell RNA sequencing of B cells to show that, in contrast to patients with severe COVID-19, subjects with mildly symptomatic COVID-19 have B cell repertoires enriched for clonally diverse, somatically-hypermutated memory B cells approximately 30 days after the onset of symptoms. This provides evidence that B cell responses are less disrupted in mild COVID-19, and result in the production of memory B cells.
登录
查看更多内容
影响因子：
64.8
作者：
Gaebler C;Wang Z;Lorenzi JCC;Muecksch F;Finkin S;Tokuyama M;Cho A;Jankovic M;Schaefer-Babajew D;Oliveira TY;Cipolla M;Viant C;Barnes CO;Bram Y;Breton G;Hägglöf T;Mendoza P;Hurley A;Turroja M;Gordon K;Millard KG;Ramos V;Schmidt F;Weisblum Y;Jha D;Tankelevich M;Martinez-Delgado G;Yee J;Patel R;Dizon J;Unson-O'Brien C;Shimeliovich I;Robbiani DF;Zhao Z;Gazumyan A;Schwartz RE;Hatziioannou T;Bjorkman PJ;Mehandru S;Bieniasz PD;Caskey M;Nussenzweig MC
通讯作者：
Nussenzweig MC
影响因子：
16.6
作者：
Zheng GX;Terry JM;Belgrader P;Ryvkin P;Bent ZW;Wilson R;Ziraldo SB;Wheeler TD;McDermott GP;Zhu J;Gregory MT;Shuga J;Montesclaros L;Underwood JG;Masquelier DA;Nishimura SY;Schnall-Levin M;Wyatt PW;Hindson CM;Bharadwaj R;Wong A;Ness KD;Beppu LW;Deeg HJ;McFarland C;Loeb KR;Valente WJ;Ericson NG;Stevens EA;Radich JP;Mikkelsen TS;Hindson BJ;Bielas JH
通讯作者：
Bielas JH
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
24.8
作者：
Kuri-Cervantes, Leticia;Pampena, Maria Betina;Betts, Michael R.
通讯作者：
Betts, Michael R.
影响因子：
5.4
作者：
Tabibian-Keissar, Hilla;Hazanov, Lena;Barshack, Iris
通讯作者：
Barshack, Iris