Evaluation of a minimal array of Treponema pallidum antigens as biomarkers for syphilis diagnosis, infection staging, and response to treatment.
Evaluation of a minimal array of Treponema pallidum antigens as biomarkers for syphilis diagnosis, infection staging, and response to treatment.
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DOI：
10.1128/spectrum.03466-23
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发表时间：
2024-01-11
影响因子：
3.7
通讯作者：
Klausner, Jeffrey D.
中科院分区：
文献类型：
--
作者：
Haynes, Austin M.;Konda, Kelika A.;Romeis, Emily;Siebert, Janet;Vargas, Silver K.;Reyes Diaz, Michael;Phan, Amber;Caceres, Carlos F.;Giacani, Lorenzo;Klausner, Jeffrey D.
梅毒是一种多阶段慢性性传播感染引起的螺旋体梅毒螺旋体亚种。苍白球（T.苍白球）。今天，梅毒仍然在低收入和中等收入国家流行，并在许多高收入国家死灰复燃。未经治疗的患者感染可导致严重的后遗症，而T。苍白球与高死产率和新生儿死亡有关。非密螺旋体和密螺旋体试验的结合允许梅毒血清学诊断和治疗反应的监测，尽管并非没有限制。在这项工作中，我们开发了一个最小的阵列，用于多个平行检测特异性抗体的免疫显性T。苍白球抗原Tp0435和Tp0574以及14种已知在感染期间具有免疫原性但仅部分或根本未评价其诊断潜力的另外的蛋白质。为了评估对这些抗原的反应性是否可以改善早期梅毒诊断，作为疾病分期的生物标志物，并监测对治疗的反应，我们使用从120名梅毒患者治疗前和治疗后纵向收集的217份血清样本测试了该阵列。结果显示，显着不同的反应，一个子集的抗原在治疗前血清（基线）时，协变量，如梅毒阶段，梅毒病史，艾滋病病毒状态的因素。此外，与基线相比，治疗后血清中对几种抗原的反应性显著降低。虽然需要更大样本组的研究来验证这些发现，但本研究支持T。梅毒蛋白质组阵列，以确定抗原，可以作为预测的反应和抗原，可以帮助早期梅毒诊断。本文探讨了宿主在感染期间对梅毒病原体的选定抗原的体液反应，以评估其作为诊断试验和治疗标记物的潜在用途。
Syphilis is a multistage chronic sexually transmitted infection caused by the spirochete Treponema pallidum subsp. pallidum (T. pallidum). Today, syphilis is still endemic in low- and middle-income nations and has become resurgent in many high-income countries. Infection can lead to severe sequelae in untreated patients, while vertical transmission of T. pallidum is associated with high stillbirth rates and neonatal death. The combination of non-treponemal and treponemal tests allows syphilis serodiagnosis and monitoring of treatment response, albeit not without limitations. In this work, we developed a minimal array for multiple parallel detection of antibodies specific to the immunodominant T. pallidum antigens Tp0435 and Tp0574 and 14 additional proteins known to be immunogenic during infection but only partially or not at all evaluated for their diagnostic potential. To assess whether reactivity to these antigens could improve early syphilis diagnosis, serve as biomarkers for disease staging, and monitor response to treatment, we tested the array using 217 serum specimens longitudinally collected pre- and post-treatment from 120 syphilis patients. Results showed significantly different reactivity to a subset of antigens in pre-treatment sera (baseline) when covariates such as syphilis stage, syphilis history, and HIV status were factored in. Furthermore, reactivity to several antigens significantly decreased in post-treatment sera compared to baseline. Although studies with larger sample panels will be needed to validate these findings, this study supports the screening of T. pallidum proteomic arrays to identify antigens that could serve as predictors of response and antigens that could help with early syphilis diagnosis. This manuscript explores the host humoral response to selected antigens of the syphilis agent during infection to evaluate their potential use as diagnostic tests and markers for treatment.
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