Folic Acid Is Able to Polarize the Inflammatory Response in LPS Activated Microglia by Regulating Multiple Signaling Pathways.
Folic Acid Is Able to Polarize the Inflammatory Response in LPS Activated Microglia by Regulating Multiple Signaling Pathways.
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DOI：
10.1155/2016/5240127
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发表时间：
2016
影响因子：
4.6
通讯作者：
Panaro MA
中科院分区：
文献类型：
--
作者：
Cianciulli A;Salvatore R;Porro C;Trotta T;Panaro MA
我们研究了叶酸对内毒素激活的BV-2小胶质细胞炎症反应的调节能力及其所涉及的信号转导途径。为此，在不同浓度的叶酸存在或不存在的情况下，将BV-2细胞系暴露于作为促炎反应诱导剂的内毒素。用Griess试验测定一氧化氮(NO)的生成。采用双抗体夹心法检测血清肿瘤坏死因子-α(TN-α)、白介素1-β(IL-1β)、白介素10(IL-10)水平。免疫印迹法检测诱导型一氧化氮合酶(INOS)、核转录因子-kappaB(NF-κB)p65、MAPKs蛋白及细胞因子信号转导抑制因子(SOCS)1和SOCS3的表达。叶酸可显著抑制脂多糖激活的BV-2细胞中肿瘤坏死因子-α和IL-1β以及诱导型一氧化氮合酶依赖性NO的产生。我们还观察到，叶酸剂量依赖性地上调BV-2细胞中SOCS1和SOCS3的表达，导致抗炎细胞因子IL-10的表达增加。叶酸可剂量依赖性地下调间接反映核因子-丝裂原活化蛋白B复合体活性的p-IκBα和κ的磷酸化水平，而叶酸可显著上调p38MAPK的磷酸化水平。总体而言，这些结果表明，叶酸能够调节小胶质细胞的炎症反应，通过调节多个信号通路来转移促炎症反应和抗炎反应。
We investigated the ability of folic acid to modulate the inflammatory responses of LPS activated BV-2 microglia cells and the signal transduction pathways involved. To this aim, the BV-2 cell line was exposed to LPS as a proinflammatory response inducer, in presence or absence of various concentrations of folic acid. The production of nitric oxide (NO) was determined by the Griess test. The levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and IL-10 were determined by ELISA. Inducible NO synthase (iNOS), nuclear transcription factor-kappa B (NF-κB) p65, MAPKs protein, and suppressors of cytokine signaling (SOCS)1 and SOCS3 were analyzed by western blotting. TNF-α and IL-1β, as well as iNOS dependent NO production, resulted significantly inhibited by folic acid pretreatment in LPS-activated BV-2 cells. We also observed that folic acid dose-dependently upregulated both SOCS1 and SOCS3 expression in BV-2 cells, leading to an increased expression of the anti-inflammatory cytokine IL-10. Finally, p-IκBα, which indirectly reflects NF-κB complex activation, and JNK phosphorylation resulted dose-dependently downregulated by folic acid pretreatment of LPS-activated cells, whereas p38 MAPK phosphorylation resulted significantly upregulated by folic acid treatment. Overall, these results demonstrated that folic acid was able to modulate the inflammatory response in microglia cells, shifting proinflammatory versus anti-inflammatory responses through regulating multiple signaling pathways.
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姜黄素消除了LPS诱导的264.7巨噬细胞中的促炎细胞因子。涉及SOCS -1，-3和P38 MAPK的新型机制的证据。
影响因子：
3
作者：
Guimaraes, Morgana Rodrigues;Manzoli Leite, Fabio Renato;Spolidorio, Luis Carlos;Kirkwood, Keith Lough;Rossa, Carlos, Jr.
通讯作者：
Rossa, Carlos, Jr.
影响因子：
5.3
作者：
Balabanov, Roumen;Strand, Krystle;Popko, Brian
通讯作者：
Popko, Brian
影响因子：
4.4
作者：
Ghosh, S
通讯作者：
Ghosh, S
影响因子：
64.5
作者：
Marine, JC;Topham, DJ;Ihle, JN
通讯作者：
Ihle, JN
DOI：
10.1007/978-1-60761-795-2_1
发表时间：
2010-01-01
期刊：
MAP KINASE SIGNALING PROTOCOLS, SECOND EDITION
影响因子：
--
作者：
Keshet, Yonat;Seger, Rony
通讯作者：
Seger, Rony