Functional interplay of histone lysine 2-hydroxyisobutyrylation and acetylation in Arabidopsis under dark-induced starvation.
Functional interplay of histone lysine 2-hydroxyisobutyrylation and acetylation in Arabidopsis under dark-induced starvation.
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黑暗饥饿下拟南芥组蛋白赖氨酸2-羟基异丁酰化和乙酰化的功能相互作用
DOI：
10.1093/nar/gkab536
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发表时间：
2021-07-21
影响因子：
14.9
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Zheng L;Li C;Ma X;Zhou H;Liu Y;Wang P;Yang H;Tamada Y;Huang J;Wang C;Hu Z;Wang X;Wang G;Li H;Hu J;Liu X;Zhou C;Zhang Y
摘要赖氨酸2-羟基异丁酰化（Khib）是一种新型的组蛋白酰化，其在植物中的发生率和功能尚不清楚。在这里，我们确定了拟南芥组蛋白上的41个Khib位点，这些位点与频繁修饰的N-尾赖氨酸（例如H3 K4、H3 K9和H4 K8）不重叠。染色质免疫沉淀测序（ChIP-seq）分析显示，35%的蛋白质编码基因中存在组蛋白Khib。大多数Khib峰位于基因区域，并且它们在转录起始位点高度富集。组蛋白Khib与乙酰化（ac）高度相关，特别是H3 K23 ac，它在基因组和基因分布上很大程度上相似。值得注意的是，组蛋白Khib和H3 K23 ac的共富集与高基因表达水平相关。代谢谱分析、转录组分析和ChIP-qPCR显示，组蛋白Khib和H3 K23 ac在参与淀粉和蔗糖代谢、戊糖和葡萄糖醛酸相互转化以及苯丙素类生物合成的基因上共同富集，并有助于微调植物对黑暗诱导的饥饿的反应。这些发现表明，Khib和H3 K23 ac可能协同作用，以促进高水平的基因转录和调节细胞代谢，以促进植物适应胁迫。最后，HDA 6和HDA 9参与去除组蛋白Khib。我们的研究结果揭示了Khib作为一种保守而独特的植物组蛋白标记，在转录相关的表观基因组过程中与赖氨酸乙酰化起作用。
Abstract Lysine 2-hydroxyisobutyrylation (Khib) is a novel type of histone acylation whose prevalence and function in plants remain unclear. Here, we identified 41 Khib sites on histones in Arabidopsis thaliana, which did not overlap with frequently modified N-tail lysines (e.g. H3K4, H3K9 and H4K8). Chromatin immunoprecipitation-sequencing (ChIP-seq) assays revealed histone Khib in 35% of protein-coding genes. Most Khib peaks were located in genic regions, and they were highly enriched at the transcription start sites. Histone Khib is highly correlated with acetylation (ac), particularly H3K23ac, which it largely resembles in its genomic and genic distribution. Notably, co-enrichment of histone Khib and H3K23ac correlates with high gene expression levels. Metabolic profiling, transcriptome analyses, and ChIP-qPCR revealed that histone Khib and H3K23ac are co-enriched on genes involved in starch and sucrose metabolism, pentose and glucuronate interconversions, and phenylpropanoid biosynthesis, and help fine-tune plant response to dark-induced starvation. These findings suggest that Khib and H3K23ac may act in concert to promote high levels of gene transcription and regulate cellular metabolism to facilitate plant adaption to stress. Finally, HDA6 and HDA9 are involved in removing histone Khib. Our findings reveal Khib as a conserved yet unique plant histone mark acting with lysine acetylation in transcription-associated epigenomic processes.
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DOI：
10.1126/science.1135862
发表时间：
2007-02-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Driscoll R;Hudson A;Jackson SP
通讯作者：
Jackson SP
影响因子：
64.5
作者：
Huang H;Sabari BR;Garcia BA;Allis CD;Zhao Y
通讯作者：
Zhao Y
影响因子：
4.4
作者：
Bergmueller, Eveline;Gehrig, Peter M.;Gruissem, Wilhelm
通讯作者：
Gruissem, Wilhelm
影响因子：
14.8
作者：
Dai, Lunzhi;Peng, Chao;Zhao, Yingming
通讯作者：
Zhao, Yingming
DOI：
10.1016/j.bbrc.2012.11.102
发表时间：
2013-03-08
影响因子：
3.1
作者：
Kim, Wanhui;Latrasse, David;Zhou, Dao-Xiu
通讯作者：
Zhou, Dao-Xiu