Zinc reduces collagen degradation in demineralized human dentin explants.
Zinc reduces collagen degradation in demineralized human dentin explants.
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DOI：
10.1016/j.jdent.2010.11.005
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发表时间：
2011-02
影响因子：
4.4
通讯作者：
Toledano M
中科院分区：
文献类型：
--
作者：
Osorio R;Yamauti M;Osorio E;Ruiz-Requena ME;Pashley DH;Tay FR;Toledano M
牙本质基质金属蛋白酶参与龋病的发病机制，并促进树脂-牙本质界面的胶原降解。目的是确定胶原蛋白降解是否可以通过过量的锌或锌chelators. METHODS矿化和磷酸脱矿的人牙本质标本进行了测试。向培养基中加入氯己定二葡萄糖酸盐、多西环素或ZnCl 2。在一半的组中，将活性外源性金属蛋白酶-2掺入溶液中。在24小时、1周和3周后进行C-末端肽测定（放射免疫法）。强力霉素完全阻断牙本质蛋白水解。氯己定二葡萄糖酸盐在24小时内降低了降解。锌过量强烈抑制胶原蛋白的水解，其效果是维持3 weeks.CONCLUSIONSZinc过量减少MMP介导的胶原蛋白降解。锌与胶原蛋白的结合导致金属蛋白酶的敏感切割位点的保护的假设需要进一步验证。
OBJECTIVESDentin matrix metalloproteinases are implicated in the pathogenesis of caries and contribute to collagen degradation in resin–dentin interfaces. The objective was to determine if collagen degradation may be modulated by an excess of zinc or zinc chelators.METHODSMineralized and phosphoric acid demineralized human dentin specimens were tested. Chlorhexidine digluconate, doxycycline or ZnCl2were added to the media. In half of the groups, active exogenous metalloproteinase-2 was incorporated into the solution. C-terminal telopeptide determinations (radioimmunoassay) were performed after 24h, 1 and 3 weeks.RESULTSCollagen degradation was prominent in demineralized dentin. Doxycycline fully blocked dentin proteolysis. Chlorhexidine digluconate reduced the degradation at the 24-h period. Zinc in excess strongly inhibits hydrolysis of collagen and its effect was maintained for 3 weeks.CONCLUSIONSZinc in excess reduces MMP-mediated collagen degradation. The hypothesis that binding of zinc to collagen results in protection of sensitive cleavage sites of metalloproteinases requires further validation.
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影响因子：
2.8
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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DOI：
10.1002/jbm.b.31295
发表时间：
2009-07
影响因子：
3.4
作者：
Carrilho, Marcela R.;Tay, Franklin R.;Donnelly, Adam M.;Agee, Kelli A.;Tjaederhane, Leo;Mazzoni, Annalisa;Breschi, Lorenzo;Foulger, Stephen;Pashley, David H.
通讯作者：
Pashley, David H.