Enzyme activities in two sister-species of carnivorous pitcher plants (Nepenthes) with contrasting nutrient sequestration strategies.
Enzyme activities in two sister-species of carnivorous pitcher plants (Nepenthes) with contrasting nutrient sequestration strategies.
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两种姊妹种食肉猪笼草（猪笼草）的酶活性与对比养分封存策略
DOI：
10.1016/j.plaphy.2021.01.049
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Bokor B
中科院分区：
文献类型：
--
作者：
Kocáb O;Bačovčinová M;Bokor B
猪笼草属的肉食性猪笼草通常吸引、捕获和消化节肢动物猎物以获得矿物质营养。但是，该属中很少有成员进化出专门的营养隔离策略，从互惠哺乳动物的粪便和尿液中获取氮，它们吸引了这些氮。因为植物以更有效的形式获得大量的氮，我们假设它们放松了消化酶的生产。如果是这样的话，消化哺乳动物粪便的物种应该比依赖节肢动物的密切相关物种表现出更少的消化酶。我们通过比较1）Hemsleyana猪笼草（其猪笼草的猪笼草是互惠的毛蝠Kerivoula hardwickii的栖息地，它也在猪笼草中排便）和2）近亲Rafflesiana猪笼草（一种典型的节肢动物捕获物种）的消化酶来验证这一假设。为了研究这两个物种中天冬氨酸蛋白酶（猪笼草蛋白酶I和II）以及III型和IV型几丁质酶的动态，我们进行了qPCR、蛋白质印迹、质谱和酶活性测量。我们发现，在猪笼草组织和消化液中的酶丰度的mRNA上调，在这两个物种的粪便和昆虫喂养。与我们最初的假设相反，最终猪笼草蛋白酶在消化液中的蛋白水解活性是更高的粪便除了比昆虫猎物无关ofNepenthesspecies。这表明，粪便可以模仿节肢动物猎物触发消化酶和N的生产。hemsleyan保留了生产这些产品的能力。
The carnivorous pitcher plants of the genusNepenthesusually attract, capture and digest arthropod prey to obtain mineral nutrients. But few members of the genus have evolved specialized nutrient sequestration strategies to acquire nitrogen from the faeces and urine of mutualistic mammals, which they attract. Because the plants obtain significant amounts of nitrogen in a more available form, we hypothesized that they have relaxed the production of digestive enzymes. If so, species that digest mammal faeces should show fewer digestive enzymes than closely related species that rely on arthropods. We tested this hypothesis by comparing digestive enzymes in 1)Nepenthes hemsleyana, whose pitchers serve as roosts for the mutualistic woolly batKerivoula hardwickii, which also defecate inside the pitchers, and 2) the close relativeNepenthes rafflesiana, a typical arthropod capturing species. To investigate the dynamics of aspartic proteases (nepenthesin I and II) and type III and IV chitinases in both species, we conducted qPCR, western blotting, mass spectrometry, and enzyme activity measurements. We found that mRNA in pitcher tissue and enzyme abundance in the digestive fluid is upregulated in both species in response to faeces and insect feeding. Contrary to our initial hypothesis, the final nepenthesin proteolytic activity in the digestive fluid is higher in response to faeces addition than to insect prey irrespective ofNepenthesspecies. This indicates that faeces can mimic arthropod prey triggering the production of digestive enzymes andN. hemsleyanaretained capacity for production of them.
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影响因子：
3.6
作者：
Wiebke Sickel;T. Grafe;I. Meuche;I. Steffan‐Dewenter;A. Keller
通讯作者：
Wiebke Sickel;T. Grafe;I. Meuche;I. Steffan‐Dewenter;A. Keller
DOI：
--
发表时间：
2015
期刊：
影响因子：
--
作者：
Yen Shan Lim;Caroline R. Schöner;Michael G. Schöner;G. Kerth;D. G. Thornham;M. Scharmann;T. Grafe
通讯作者：
T. Grafe
肉食性猪笼草植物的粪食特征：脲酶的任务
影响因子：
4.6
作者：
Ayufu Yilamujiang;Anting Zhu;R. Ligabue;S. Bartram;C. Witte;R. Hedrich;Mitsuyasu Hasabe;Caroline R. Schöner;Michael G. Schöner;G. Kerth;C. Carlini;A. Mithöfer
通讯作者：
A. Mithöfer
影响因子：
2.8
作者：
W. W. Wan Zakaria-W.;W. Aizat;Hoe-Han Goh;Normah Mohd Noor
通讯作者：
W. W. Wan Zakaria-W.;W. Aizat;Hoe-Han Goh;Normah Mohd Noor
DOI：
10.1046/j.1365-313x.1999.00414.x
发表时间：
1999-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Waltraud X. Schulze;Wolf B. Frommer;John M. Ward
通讯作者：
Waltraud X. Schulze;Wolf B. Frommer;John M. Ward