A unique approach toward near-infrared fluorescent probes for bioimaging with remarkably enhanced contrast.
A unique approach toward near-infrared fluorescent probes for bioimaging with remarkably enhanced contrast.
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用于生物成像的近红外荧光探针的独特方法，对比度显着增强
DOI：
10.1039/c5sc04014k
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发表时间：
2016-03-01
期刊：
影响因子：
8.4
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Gong YJ;Zhang XB;Mao GJ;Su L;Meng HM;Tan W;Feng S;Zhang G
在生物成像应用中具有显著增强的图像对比度和表观亮度的通用传感平台。近红外荧光探针具有自发荧光干扰小、组织穿透深、对样品损伤小等优点，是生物成像的重要工具。然而，大多数以前报道的近红外探针表现出小的斯托克斯位移，通常小于30 nm，和低的荧光量子产率，严格限制的对比度和空间分辨率的生物成像。在此，通过扩展罗丹明B的π共轭体系，同时保持其刚性和平面结构，我们报道了一种高效的近红外染料HN 7，其在乙醇中具有73 nm的大斯托克斯位移和高达0.72的荧光量子产率，其值上级优于传统的菁类近红外染料如Cy 5。使用HN 7，活细胞，组织和小鼠进行成像，结果显示与Cy 5相比，对比度显著增强，空间分辨率提高，组织成像深度令人满意。此外，罗丹明B的非荧光螺环结构是HN 7的固有组成部分，因此，我们的策略提供了一个通用的平台，设计高效的近红外开启生物成像探针的各种目标。作为概念验证，分别设计、合成了两种不同的用于NO和Hg 2+的近红外探针HN 7-N2和HN 7-S，并分别成功地应用于活细胞、组织和小鼠中的NO和Hg 2+的成像，展示了新探针的潜在生物成像应用。总之，这种新型染料可能为开发用于生物应用中的对比度增强成像的高效NIR荧光探针提供新的途径。
A universal sensing platform with significantly enhanced image contrast and apparent brightness in biological imaging applications. Near-infrared (NIR) fluorescent probes are attractive molecular tools for bioimaging because of their low autofluorescence interference, deep tissue penetration, and minimal damage to sample. However, most previously reported NIR probes exhibit small Stokes shift, typically less than 30 nm, and low fluorescence quantum yield, strictly limited contrast and spatial resolution for bioimaging. Herein, by expanding the π-conjugated system of rhodamine B, while, at the same time, keeping its rigid and planar structure, we reported an efficient NIR dye, HN7, with large stokes shift of 73 nm and fluorescence quantum yield as high as 0.72 in ethanol, values superior to those of such traditional cyanine NIR dyes as Cy5. Using HN7, living cells, tissues and mice were imaged, and the results showed significantly enhanced contrast, improved spatial resolution, and satisfactory tissue imaging depth when compared to Cy5. Moreover, the nonfluorescent spirocyclic structure of rhodamine B is an inherent component of HN7; therefore, our strategy provided a universal platform for the design of efficient NIR turn-on bioimaging probes for various targets. As a proof-of-concept, two different NIR probes, HN7-N2 and HN7-S for NO and Hg2+, respectively, were designed, synthesized, and successfully applied for the imaging of NO and Hg2+ in living cells, tissues and mice, respectively, demonstrating the potential bioimaging applications of the new probes. In sum, this new type of dye may present new avenues for the development of efficient NIR fluorescent probes for contrast-enhanced imaging in biological applications.
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影响因子：
4.7
作者：
McCann, Thomas E.;Kosaka, Nobuyuki;Koide, Yuichiro;Mitsunaga, Makoto;Choyke, Peter L.;Nagano, Tetsuo;Urano, Yasuteru;Kobayashi, Hisataka
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影响因子：
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作者：
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作者：
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通讯作者：
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15
作者：
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Shabat, Doron
基于半花青的高分辨率比率近红外荧光探针，用于监测体内 pH 值变化
影响因子：
7.4
作者：
Li, Yinhui;Wang, Yijun;Yang, Ronghua
通讯作者：
Yang, Ronghua