Macromolecular fingerprinting of sulfolobus species in biofilm: a transcriptomic and proteomic approach combined with spectroscopic analysis.
Macromolecular fingerprinting of sulfolobus species in biofilm: a transcriptomic and proteomic approach combined with spectroscopic analysis.
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DOI：
10.1021/pr2003006
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发表时间：
2011-09-02
影响因子：
4.4
通讯作者：
Albers, Sonja-Verena
中科院分区：
文献类型：
--
作者：
Koerdt, Andrea;Orell, Alvaro;Trong Khoa Pham;Mukherjee, Joy;Wlodkowski, Alexander;Karunakaran, Esther;Biggs, Catherine A.;Wright, Phillip C.;Albers, Sonja-Verena
自然界中的微生物通常生活在表面相关的固着群落中，包裹在自己产生的基质中，称为生物膜。生物膜在细菌中得到了很好的研究，但对古生菌的研究有限。最近，我们对三种关系密切的嗜热古生物的生物膜形成进行了描述：Sulfolobus acidocaldarius、S.solfararicus和S.tokodaii。这些菌株在静态条件下形成了不同的群落，从索氏链球菌的简单地毯结构到酸奶链球菌的高密度塔状结构。在这里，我们结合光谱学、蛋白质组学和转录组学分析来描述与生物膜相关的生理和调节特征。光谱分析表明，与浮游生活方式相比，生物膜生活方式对硫叶藻的生理有明显的影响。蛋白质组学和转录组数据表明，生物膜形成的生活方式是菌株特有的(例如，大约15%的酸链球菌基因有不同的表达，索尔法氏链球菌和东京链球菌的∼分别为3.4%和∼1%)。基因组数据表明，受调控的ORF广泛分布于细胞的基本功能中，包括表面修饰。在所有三个物种中，生物膜形成细胞都有几个共同的受调控基因。最显著的共同反应基因之一包括推定的Lrs14类转录调节因子，表明它们可能是生物膜发育中的关键调控因素。酸链霉菌、立枯链球菌和东京链霉菌作为生物膜独立生长。通过光谱分析(FTIR和XPS)、iTRAQ蛋白质组学和RNA微阵列对三个菌株的浮游细胞和生物膜细胞突起进行了比较。为了突出Sulfolobus菌株在生物膜形成方面的共同特征，提供了这些数据作为比较分析。最显著的共同反应基因之一包括推测的Lrs14类转录调节因子，表明它们在生物膜发育中发挥关键调控因素的作用。
Microorganisms in nature often live in surface-associated sessile communities, encased in a self-produced matrix, referred to as biofilms. Biofilms have been well studied in bacteria but in a limited way for archaea. We have recently characterized biofilm formation in three closely related hyperthermophilic crenarchaeotes: Sulfolobus acidocaldarius, S. solfataricus, and S. tokodaii. These strains form different communities ranging from simple carpet structures in S. solfataricus to high density tower-like structures in S. acidocaldarius under static condition. Here, we combine spectroscopic, proteomic, and transcriptomic analyses to describe physiological and regulatory features associated with biofilms. Spectroscopic analysis reveals that in comparison to planktonic life-style, biofilm life-style has distinctive influence on the physiology of each Sulfolobus spp. Proteomic and transcriptomic data show that biofilm-forming life-style is strain specific (eg ca. 15% of the S. acidocaldarius genes were differently expressed, S. solfataricus and S. tokodaii had ∼3.4 and ∼1%, respectively). The -omic data showed that regulated ORFs were widely distributed in basic cellular functions, including surface modifications. Several regulated genes are common to biofilm-forming cells in all three species. One of the most striking common response genes include putative Lrs14-like transcriptional regulators, indicating their possible roles as a key regulatory factor in biofilm development. S. acidocaldarius, S. solfataricus, and S. tokodaii strains were grown independently as biofilms. Comparison between planktonic and biofilm cell popupations of all three strains was performed by spectroscopic analysis (FTIR and XPS), iTRAQ proteomics, and RNA microarrays. To highlight common features in biofilm formation among the Sulfolobus strains, the data is presented as a comparative analysis. One of the most striking common response genes include putative Lrs14-like transcriptional regulators, suggesting their roles as key regulatory factor in biofilm development.
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影响因子：
2.9
作者：
Clarke, David J.;Ortega, Ximena P.;Brown, Alan R.
通讯作者：
Brown, Alan R.
影响因子：
3.2
作者：
Koo, H.;Xiao, J.;Jeon, J. G.
通讯作者：
Jeon, J. G.
影响因子：
5
作者：
Bosch, A.;Serra, D.;Yantorno, O.
通讯作者：
Yantorno, O.
影响因子：
4.4
作者：
Auernik, Kathryne S.;Maezato, Yukari;Kelly, Robert M.
通讯作者：
Kelly, Robert M.
影响因子：
4.4
作者：
Lebeer, Sarah;Verhoeven, Tine L. A.;De Keersmaecker, Sigrid C. J.
通讯作者：
De Keersmaecker, Sigrid C. J.