Insulin gene expression in chicken ontogeny: pancreatic, extrapancreatic, and prepancreatic.
Insulin gene expression in chicken ontogeny: pancreatic, extrapancreatic, and prepancreatic.
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鸡个体发育中的胰岛素基因表达：胰腺、胰腺外和胰腺前。
DOI：
10.1016/0012-1606(89)90237-6
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发表时间：
1989
影响因子：
2.7
通讯作者：
F. Pablo
中科院分区：
文献类型：
--
作者：
Jose Serrano;Charles L Bevins;Scott W. Young;F. Pablo
胰岛素在鸡胚胎活体内具有代谢、生长和分化作用，是正常发育所必需的。胰腺是否是胚胎发育过程中胰岛素的唯一来源仍存在争议。在本研究中，我们研究了(1)鸡胰岛素基因在胰腺中表达的发育模式；(2)胰岛素基因在鸡发育过程中肝、脑和肢体三个非胰腺组织中的表达；(3)胰岛素基因在胰腺前期和鸡胚器官发生过程中的表达。合成的物种特异性胰岛素寡核苷酸与胰腺冰冻切片和Northern杂交显示，在胚胎发育的第三周，胰岛素信使RNA(MRNA)水平显著增加。杂交组织化学显示，随着发育的发展，每个胰岛的胰岛素mRNA水平增加，同时含有胰岛素mRNA的胰岛数目也增加。Northern分析显示有一个0.6kb的主要多聚腺苷化转录本，在此期间丰度增加了约30倍。在与胰腺RNA相同的严格条件下，在肝脏RNA印迹中检测到胰岛素转录本。肝脏胰岛素mRNA的丰度比胰腺胰岛素mRNA的丰度低约100倍，与后者相反，在发育后期不会增加。引物延伸实验表明，胰腺和肝脏的胰岛素转录本具有相似的5‘端。Northern分析和引物延伸均未检测到不同发育阶段的全脑或肢体总RNA中的胰岛素基因。在胰腺器官发生的第8天，整个胚胎和第4天和第5天的身体部位都检测到极低丰度的胰岛素mRNA。有趣的是，在β细胞分化之前，在整个第二天和第三天的胚胎(阶段10到20，有20到40个体节)中也检测到了多腺化胰岛素转录本。因此，胰岛素基因在几种鸡胚组织中存在不同的发育调控，并且胰岛素的表达先于胰腺成熟。这些发现支持胰岛素在活体分化和发育中的作用，并表明在血液循环开始之前，胰岛素在早期胚胎中的旁分泌类型的作用。
Insulin has metabolic, growth, and differentiation effects in chicken embryosin vivoand it is required for normal development. Whether the pancreas is the sole source of insulin in embryogenesis is controversial. In the present study we investigated (1) the developmental pattern of expression of the chicken insulin gene in the pancreas; (2) the expression of the insulin gene in three nonpancreatic tissues, liver, brain, and lower limb, during chicken development; and (3) the expression of the insulin gene at prepancreatic stages and during chicken embryo organogenesis. Hybridization of synthetic species-specific insulin oligonucleotides to pancreatic frozen sectionin situand to Northern blots revealed a major increase in insulin messenger RNA (mRNA) levels during the third week of embryonic development. The hybridization histochemistry showed both an increase in the levels of insulin mRNA per pancreatic islet and, in addition, an increase in the number of insulin mRNA containing islets with development. By Northern analysis there was a major polyadenylated transcript of 0.6 kb, which increased in abundance approximately 30-fold during this interval. Under the same stringency conditions used for pancreatic RNA an insulin transcript was detected in liver RNA blots. The abundance of this hepatic insulin mRNA was about 100-fold less than the pancreatic insulin mRNA and, in contrast to the latter, did not increase in late development. Primer extension experiments demonstrated that the insulin transcripts of pancreas and liver had similar 5′ ends. No insulin mRNA was detected by Northern analysis or primer extension either in whole brain or lower limb total RNA from several developmental stages. A very low abundance insulin mRNA was detected in whole embryo at Day 8 and body regions at Day 4 and Day 5 when organogenesis of the pancreas takes place. Interestingly, a polyadenylated insulin transcript was detected, as well, in whole Day 2 and Day 3 embryos (stages 10 to 20, with 20 to 40 somites) before differentiation of β cells occurs. Thus, there is differential developmental regulation of the insulin gene in several chicken embryo tissues and the expression of insulin preceeds pancreatic maturation. These findings support the proposed role of insulin in differentiation and developmentin vivoand suggest a paracrine type of action of the hormone in early embryos before blood circulation begins.
生长调节素和胰岛素对体外成肌细胞分化的影响。
影响因子：
2.7
作者：
Ewton,DZ;Florini,JR
通讯作者：
Florini,JR
DOI：
10.1073/pnas.81.12.3635
发表时间：
1984
影响因子：
11.1
作者：
Muglia,L;Locker,J
通讯作者：
Locker,J
DOI：
10.1089/dna.1983.2.329
发表时间：
1983-01-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
--
作者：
CATHALA, G;SAVOURET, JF;BAXTER, JD
通讯作者：
BAXTER, JD