Effects of the somatomedins and insulin on myoblast differentiation in vitro.
Effects of the somatomedins and insulin on myoblast differentiation in vitro.
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生长调节素和胰岛素对体外成肌细胞分化的影响。
DOI：
10.1016/0012-1606(81)90312-2
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发表时间：
1981
影响因子：
2.7
通讯作者：
Florini,JR
中科院分区：
文献类型：
--
作者：
Ewton,DZ;Florini,JR
本文以Yaffe ′ s L 6细胞系为实验材料，研究了胰岛素和生长调节素对大鼠成肌细胞分化的影响。无论是胰岛素或Temin的增殖刺激活性（MSA），生长调节蛋白家族的成员，孵育48小时，引起成肌细胞融合的显着增加。这种分化的刺激不是这些激素对成肌细胞增殖的作用导致的细胞密度增加的简单结果，并且融合的增加不是所有有丝分裂原的共同作用（FGF抑制该过程）。其他生长调节素（人生长调节素C和胰岛素样生长因子I）在刺激分化方面与MSA一样有效。生长调节素在胎儿血液中的浓度范围内具有活性，而胰岛素在浓度低于10− 7，M时无活性。生长激素（GH）对肌肉分化无影响。在无血清培养基MM-1（其中成肌细胞保持明显正常的形态和代谢活性）中，刺激分化所需的极高水平的胰岛素可以完全被生理水平（1.0 μg/ml）的MSA所取代，进一步支持了我们的观点，即高浓度胰岛素主要作为生长调节素的类似物刺激肌细胞的生长和发育。
The effects of insulin and the somatomedins on differentiation of rat myoblasts were investigated in experiments on cells cloned from Yaffe's L6 line. Incubation for 48 hr with either insulin or Temin's multiplication stimulating activity (MSA), a member of the somatomedin family, caused a dramatic increase in myoblast fusion. This stimulation of differentiation is not a simple consequence of the increased cell density resulting from the effects of these hormones on myoblast proliferation, and the increase in fusion is not an effect common to all mitogens (FGF inhibits the process). Other somatomedins (human somatomedin C and insulin-like growth factor I), were as effective as MSA in stimulating differentiation. The somatomedins were active at concentrations in the range of their levels in fetal blood, in contrast to insulin, which was inactive at concentrations below 10−7,M. Growth hormone (GH) had no effect on muscle differentiation. In serum-free medium MM-1 (in which myoblasts maintain apparently normal morphology and metabolic activity), the very high levels of insulin required to stimulate differentiation could be replaced entirely by physiological levels (1.0 μg/ml) of MSA, further supporting our view that insulin at high concentrations serves primarily as an analogue of the somatomedins in stimulating the growth and development of muscle cells.
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影响因子：
2.7
作者：
M. Kumegawa;E. Ikeda;S. Hosoda;T. Takuma
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DOI：
10.1210/jcem-46-4-672
发表时间：
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期刊：
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影响因子：
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作者：
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C. J. Johnson
DOI：
--
发表时间：
1967
期刊：
Journal of Laboratory and Clinical Medicine
影响因子：
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作者：
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影响因子：
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作者：
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作者：
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