Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining.
Visualization of cortical microtubule networks in plant cells by live imaging and immunostaining.
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DOI：
10.1016/j.xpro.2021.100301
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Jiao Y
中科院分区：
文献类型：
--
作者：
Du F;Zhao F;Traas J;Jiao Y
皮质微管(CMTs)在植物细胞生长和分裂过程中起着关键作用。CMTs的组织在不同的细胞和发育过程中经历了重要的变化。在这里，我们描述了两种利用共聚焦激光扫描显微镜显示植物细胞中CMT组织的方法。通过荧光报告线的实时成像可以直接显示外层组织中的CMT网络，切片和免疫染色相结合的方案被应用于整个组织中的CMT的可视化。有关本议定书的使用和执行的完整细节，请参阅)。植物组织外层皮质微管的活体成像方法琼脂糖凝胶包埋切片法暴露植物内部细胞皮质微管免疫组织化学染色方法皮质微管(CMTs)在植物细胞的生长和分裂过程中起着关键作用。CMTs的组织在不同的细胞和发育过程中经历了重要的变化。在这里，我们描述了两种利用共聚焦激光扫描显微镜显示植物细胞中CMT组织的方法。通过荧光报告线的实时成像可以直接显示外层组织中的CMT网络，切片和免疫染色相结合的方案被应用于整个组织中的CMT的可视化。
Cortical microtubules (CMTs) play pivotal roles during plant cell growth and division. The organization of CMTs undergoes important changes during different cellular and developmental processes. Here, we describe two methods for the visualization of CMT organization in plant cells using confocal laser scanning microscopy. CMT networks in the outer tissue layers can be directly visualized by live imaging of a fluorescent reporter line, and a protocol combining sectioning and immunostaining is applied for visualization of CMTs throughout tissues. For complete details on the use and execution of this protocol, please refer to). Methods for live imaging of cortical microtubules in outer cell layers of plant tissues Agarose-gel embedding and sectioning method for exposing inner plant cells Methods for immunostaining of cortical microtubules in inner cells of plant tissues Cortical microtubules (CMTs) play pivotal roles during plant cell growth and division. The organization of CMTs undergoes important changes during different cellular and developmental processes. Here, we describe two methods for the visualization of CMT organization in plant cells using confocal laser scanning microscopy. CMT networks in the outer tissue layers can be directly visualized by live imaging of a fluorescent reporter line, and a protocol combining sectioning and immunostaining is applied for visualization of CMTs throughout tissues.
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影响因子：
7.7
作者：
Barbier de Reuille P;Routier-Kierzkowska AL;Kierzkowski D;Bassel GW;Schüpbach T;Tauriello G;Bajpai N;Strauss S;Weber A;Kiss A;Burian A;Hofhuis H;Sapala A;Lipowczan M;Heimlicher MB;Robinson S;Bayer EM;Basler K;Koumoutsakos P;Roeder AH;Aegerter-Wilmsen T;Nakayama N;Tsiantis M;Hay A;Kwiatkowska D;Xenarios I;Kuhlemeier C;Smith RS
通讯作者：
Smith RS
影响因子：
56.9
作者：
Hamant, Olivier;Heisler, Marcus G.;Traas, Jan
通讯作者：
Traas, Jan
影响因子：
14.8
作者：
Boudaoud, Arezki;Burian, Agata;Hamant, Olivier
通讯作者：
Hamant, Olivier
影响因子：
7.2
作者：
de Reuille, PB;Bohn-Courseau, I;Traas, J
通讯作者：
Traas, J
DOI：
10.1016/j.cub.2020.07.076
发表时间：
2020-10-19
期刊：
Current biology : CB
影响因子：
--
作者：
Zhao F;Du F;Oliveri H;Zhou L;Ali O;Chen W;Feng S;Wang Q;Lü S;Long M;Schneider R;Sampathkumar A;Godin C;Traas J;Jiao Y
通讯作者：
Jiao Y