Calcineurin B-Like family in Populus: comparative genome analysis and expression pattern under cold, drought and salt stress treatment
Calcineurin B-Like family in Populus: comparative genome analysis and expression pattern under cold, drought and salt stress treatment
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杨属钙调神经磷酸酶 B 样家族：寒冷、干旱和盐胁迫处理下的比较基因组分析和表达模式
DOI：
10.1007/s10725-008-9293-4
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发表时间：
2008-06
影响因子：
4.2
通讯作者：
Yin, Weilun
中科院分区：
文献类型：
--
作者：
Zhang, Hechen;Xia, Xinli;Yin, Weilun
钙在植物的非生物胁迫反应中起着重要的调节作用。类钙调神经磷酸酶(CBL)蛋白是拟南芥和水稻钙信号通路中的钙感受器。然而，钙信号转导的分子机制仍有待阐明，尤其是在杨树中，杨树是一种具有完整基因组序列的模式树种。在此，我们对杨树基因组中的10个CBL候选基因进行了特征分析，并对杨树、水稻和拟南芥中的CBL基因进行了比较基因组学分析，包括基因结构、保守基序和系统发育。比较基因组学分析表明，杨树中的家系在大小和结构上比较保守，重复事件可能对该基因家族的扩增有显著的贡献。此外，为了阐明CBL基因成员在调节植物对外界刺激的反应中各自的功能，我们对主要耐盐和干旱的胡杨CBL家族进行了研究。从胡杨中分离到9个PeCBL成员的基因克隆，并用基因特异性RT-PCR方法研究了它们在非生物胁迫(如寒冷、干旱和盐分)处理下的表达模式。对非生物胁迫下胡杨CBL基因的转录分析表明，7个CBL基因成员(PeCBL1、2、3、4、5、9和10)可能在特定外界刺激的响应中发挥重要作用。我们的数据将为进一步研究植物CBL信号网络的功能解剖提供重要证据。
Calcium plays a critical role in regulating abiotic-stress response in plant. Calcineurin B-like (CBL) proteins have been identified as calcium sensors in the calcium signaling pathway in Arabidopsis and rice. However, molecular mechanism underlying calcium signaling remains to be elucidated, especially in poplar, a model tree species with complete sequencing of the whole genome. Here, we have characterized 10 CBL candidate genes in the genome of Populustrichocarpa (PtCBLs) and presented a comparative genomics analysis of CBLs in poplar, rice and Arabidopsis, including gene structures, conserved motifs and phylogeny. Comparative genomics analyses showed that the family in poplar appears to be rather conserved in the size and structure, and the duplication events might significantly contribute to the amplification of this gene family. Furthermore, to elucidate the functions of CBL gene members respectively in regulating plant response to external stimuli, we presented a study of CBL family in Populus euphratica (PeCBLs), a mostly salt- and drought-tolerant Populus species. Nine cDNA clones of PeCBL members were isolated from Populus euphratica and a gene specific RT-PCR was performed to investigate their expression patterns under abiotic stress treatment, such as cold, drought, and salt. A transcript analysis of CBLs in Populus euphratica under abiotic stress suggests that seven CBL gene (PeCBL1, 2, 3, 4, 5, 9, and 10) members may play an important role in correspondence to specific external stimuli. Our data will provide important evidence for further studying the functional dissection of the CBL signaling network in plant.
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影响因子：
4.8
作者：
Nagae, M;Nozawa, A;Shimizu, T
通讯作者：
Shimizu, T
影响因子：
11.4
作者：
V. Kidd;T. Lion
通讯作者：
V. Kidd;T. Lion
DOI：
10.1073/pnas.97.7.3730
发表时间：
2000-03
影响因子：
11.1
作者：
Jiping Liu;M. Ishitani;U. Halfter;Cheol-Soo Kim;Jian‐Kang Zhu
通讯作者：
Jiping Liu;M. Ishitani;U. Halfter;Cheol-Soo Kim;Jian‐Kang Zhu
影响因子：
11.6
作者：
Quan, Ruidang;Lin, Huixin;Guo, Yan
通讯作者：
Guo, Yan
影响因子：
7.4
作者：
Pandey, GK;Grant, JJ;Luan, S
通讯作者：
Luan, S