Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties.
Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties.
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DOI：
10.1080/19490976.2021.1983101
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发表时间：
2021-01
期刊：
影响因子：
12.2
通讯作者：
Chen MS
中科院分区：
文献类型：
--
作者：
Halimi A;Gabarrini G;Sobkowiak MJ;Ateeb Z;Davanian H;Gaiser RA;Arnelo U;Valente R;Wong AYW;Moro CF;Del Chiaro M;Özenci V;Chen MS
新兴研究表明肠道微生物组可能在胰腺癌的发生和进展中发挥作用，但癌症微生物组的培养仍然具有挑战性。这项试点研究旨在调查从与侵袭性癌症相关的胰腺囊性病变中培养胰腺微生物组的可能性。术中采集的胰腺囊肿液样本主要在导管内乳头状粘液性肿瘤（IPMN）病变组中显示培养阳性。 MALDI-TOF MS 分析显示，Gammaproteobacteria 和 Bacilli 在单个细菌分离株中占主导地位。在培养的细菌中，γ变形菌，特别是肺炎克雷伯菌，还有颗粒菌和粪肠球菌，在离体共培养模型中测试的胰腺细胞系中表现出一致的致病特性。致病特性包括细胞内存活能力、细胞死亡诱导或在存活细胞中引起DNA双链断裂，类似于基因毒性效应。这项研究为胰腺微生物群在胰腺囊性病变和癌症之间的有趣联系中的作用提供了新的见解。
Emerging research suggests gut microbiome may play a role in pancreatic cancer initiation and progression, but cultivation of the cancer microbiome remains challenging. This pilot study aims to investigate the possibility to cultivate pancreatic microbiome from pancreatic cystic lesions associated with invasive cancer. Intra-operatively acquired pancreatic cyst fluid samples showed culture-positivity mainly in the intraductal papillary mucinous neoplasm (IPMN) group of lesions. MALDI-TOF MS profiling analysis shows Gammaproteobacteria and Bacilli dominate among individual bacteria isolates. Among cultivated bacteria, Gammaproteobacteria, particularly Klebsiella pneumoniae, but also Granulicatella adiacens and Enterococcus faecalis, demonstrate consistent pathogenic properties in pancreatic cell lines tested in ex vivo co-culture models. Pathogenic properties include intracellular survival capability, cell death induction, or causing DNA double-strand breaks in the surviving cells resembling genotoxic effects. This study provides new insights into the role of the pancreatic microbiota in the intriguing link between pancreatic cystic lesions and cancer.
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影响因子：
24.5
作者：
Felsenstein, Matthaus;Noe, Michael;Masica, David L.;Hosoda, Waki;Chianchiano, Peter;Fischer, Catherine G.;Lionheart, Gemma;Brosens, Lodewijk A. A.;Pea, Antonio;Yu, Jun;Gemenetzis, Georgios;Groot, Vincent P.;Makary, Martin A.;He, Jin;Weiss, Matthew J.;Cameron, John L.;Wolfgang, Christopher L.;Hruban, Ralph H.;Roberts, Nicholas J.;Karchin, Rachel;Goggins, Michael G.;Wood, Laura D.
通讯作者：
Wood, Laura D.
影响因子：
24.5
作者：
European Study Group on Cystic Tumours of the Pancreas
通讯作者：
European Study Group on Cystic Tumours of the Pancreas
影响因子：
1.6
作者：
Flanagan, Dennis
通讯作者：
Flanagan, Dennis
影响因子：
3.7
作者：
Cameron SJS;Lewis KE;Huws SA;Hegarty MJ;Lewis PD;Pachebat JA;Mur LAJ
通讯作者：
Mur LAJ
DOI：
10.1073/pnas.1820594116
发表时间：
2019-04-23
影响因子：
11.1
作者：
Baker, Jonathon L.;Hendrickson, Erik L.;Shi, Wenyuan
通讯作者：
Shi, Wenyuan