Genomic analysis of carbon monoxide utilization and butanol production by Clostridium carboxidivorans strain P7.
Genomic analysis of carbon monoxide utilization and butanol production by Clostridium carboxidivorans strain P7.
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DOI：
10.1371/journal.pone.0013033
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发表时间：
2010-09-27
期刊：
影响因子：
3.7
通讯作者：
Masson L
中科院分区：
文献类型：
--
作者：
Bruant G;Lévesque MJ;Peter C;Guiot SR;Masson L
对生产可再生燃料的需求不断增加，最近对丁醇的微生物生产产生了特别的兴趣。厌氧细菌，如梭菌属，可以自然地将碳水化合物转化为各种初级产品，包括丁醇等醇类。微生物如丙酮丁醇梭菌的遗传学已经得到了很好的研究，其产生溶剂的代谢途径的特点。相比之下，对梭菌属的遗传学知之甚少。能够将合成气或其单独组分转化为溶剂。本研究对一种新的产溶剂梭菌C. carboxidivorans的基因特征通过基因组测序。C. Carboxidivorans菌株P7 T具有完整的Wood-Ljungdahl途径基因簇，涉及CO和CO2固定并转化为乙酰辅酶A。此外，除了丙酮生产途径，所有的经典ABE代谢途径的乙酸，丁酸，乙醇和丁醇生产的遗传决定因素都存在于P7 T染色体。这些途径的功能性也通过P7 T在CO上的生长和CO2以及挥发性脂肪酸（乙酸酯和丁酸酯）和溶剂（乙醇和丁醇）的产生来证实。还发现P7 T含有一个19 Kbp的质粒，该质粒不包括必需基因或丁醇生产相关基因。本研究对P7 T基因组有了深入的了解，这将有助于开发代谢工程策略以改善C。carboxidivorans的天然能力，生产潜在的生物燃料的合成气。
Increasing demand for the production of renewable fuels has recently generated a particular interest in microbial production of butanol. Anaerobic bacteria, such as Clostridium spp., can naturally convert carbohydrates into a variety of primary products, including alcohols like butanol. The genetics of microorganisms like Clostridium acetobutylicum have been well studied and their solvent-producing metabolic pathways characterized. In contrast, less is known about the genetics of Clostridium spp. capable of converting syngas or its individual components into solvents. In this study, the type of strain of a new solventogenic Clostridium species, C. carboxidivorans, was genetically characterized by genome sequencing. C. carboxidivorans strain P7T possessed a complete Wood-Ljungdahl pathway gene cluster, involving CO and CO2 fixation and conversion to acetyl-CoA. Moreover, with the exception of an acetone production pathway, all the genetic determinants of canonical ABE metabolic pathways for acetate, butyrate, ethanol and butanol production were present in the P7T chromosome. The functionality of these pathways was also confirmed by growth of P7T on CO and production of CO2 as well as volatile fatty acids (acetate and butyrate) and solvents (ethanol and butanol). P7T was also found to harbour a 19 Kbp plasmid, which did not include essential or butanol production related genes. This study has generated in depth knowledge of the P7T genome, which will be helpful in developing metabolic engineering strategies to improve C. carboxidivorans's natural capacity to produce potential biofuels from syngas.
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影响因子：
8.4
作者：
Desai, Ruchir P.;Harris, Latonia M.;Papoutsakis, Eleftherios T.
通讯作者：
Papoutsakis, Eleftherios T.
DOI：
10.1099/00207713-51-6-2095
发表时间：
2001-11-01
影响因子：
2.8
作者：
Keis, S;Shaheen, R;Jones, DT
通讯作者：
Jones, DT
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
4.4
作者：
Boynton, ZL;Bennett, GN;Rudolph, FB
通讯作者：
Rudolph, FB
影响因子：
4.4
作者：
GEORGE, HA;CHEN, JS
通讯作者：
CHEN, JS