TRUE Gene Silencing.
TRUE Gene Silencing.
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DOI：
10.3390/ijms23105387
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发表时间：
2022-05-11
影响因子：
5.6
通讯作者：
Nashimoto, Masayuki
中科院分区：
文献类型：
--
作者：
Nashimoto, Masayuki
TRUE基因沉默是一种rna介导的基因表达控制技术，以tRNase zl命名，利用有效的基因沉默。在这篇综述中，我概述了小向导RNA （sgRNA）作为TRUE基因沉默的新疗法的潜力。首先，我介绍了tRNase ZL和细胞小RNA的生理，然后是sgRNA和TRUE基因沉默。核糖核酸内切酶tRNase ZL可以有效地从前tRNA中去除3 '尾链，被认为在细胞核和线粒体中tRNA成熟中起作用。在活细胞中存在多种具有sgRNA功能的小rna，包括miRNA和tRNA、rRNA片段，人类细胞似乎与这些小rna一起利用胞质tRNase ZL进行基因调控。利用tRNase ZL识别和切割micro-pre-tRNA、pre-tRNA样复合物或micro-pre-tRNA样复合物以及pre-tRNA的特性，在人工sgRNA结合靶RNA形成pre-tRNA样复合物或micro-pre-tRNA样复合物的指导下，tRNase ZL可以在任何需要的位点切割任何靶RNA。这种通用的RNA切割方法是TRUE基因沉默的基础。本文综述了TRUE基因沉默的各种应用实例，包括在几种人类癌细胞中的应用，以诱导细胞凋亡。最后，我讨论了sgRNA作为多发性骨髓瘤新疗法的潜力。
TRUE gene silencing is an RNA-mediated gene expression control technology and is termed after tRNase ZL-utilizing efficacious gene silencing. In this review, I overview the potentiality of small guide RNA (sgRNA) for TRUE gene silencing as novel therapeutics. First, I describe the physiology of tRNase ZL and cellular small RNA, and then sgRNA and TRUE gene silencing. An endoribonuclease, tRNase ZL, which can efficiently remove a 3′ trailer from pre-tRNA, is thought to play the role in tRNA maturation in the nucleus and mitochondria. There exist various small RNAs including miRNA and fragments from tRNA and rRNA, which can function as sgRNA, in living cells, and human cells appear to be harnessing cytosolic tRNase ZL for gene regulation together with these small RNAs. By utilizing the property of tRNase ZL to recognize and cleave micro-pre-tRNA, a pre-tRNA-like or micro-pre-tRNA-like complex, as well as pre-tRNA, tRNase ZL can be made to cleave any target RNA at any desired site under the direction of an artificial sgRNA that binds a target RNA and forms the pre-tRNA-like or micro-pre-tRNA-like complex. This general RNA cleavage method underlies TRUE gene silencing. Various examples of the application of TRUE gene silencing are reviewed including the application to several human cancer cells in order to induce apoptosis. Lastly, I discuss the potentiality of sgRNA as novel therapeutics for multiple myeloma.
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影响因子：
11.4
作者：
Chim CS;Kumar SK;Orlowski RZ;Cook G;Richardson PG;Gertz MA;Giralt S;Mateos MV;Leleu X;Anderson KC
通讯作者：
Anderson KC
影响因子：
3.7
作者：
Takahashi M;Elbarbary RA;Abe M;Sato M;Yoshida T;Yamada Y;Tamura M;Nashimoto M
通讯作者：
Nashimoto M
影响因子：
3.7
作者：
Elbarbary RA;Takaku H;Uchiumi N;Tamiya H;Abe M;Takahashi M;Nishida H;Nashimoto M
通讯作者：
Nashimoto M
影响因子：
14.9
作者：
Nashimoto, M
通讯作者：
Nashimoto, M
影响因子：
14.9
作者：
Deprey, Kirsten;Batistatou, Nefeli;Kritzer, Joshua A.
通讯作者：
Kritzer, Joshua A.